Development of a replicative plasmid for gene expression in Mycoplasma bovis
Development of a replicative plasmid for gene expression in Mycoplasma bovis
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DOI：
10.1016/j.mimet.2014.11.005
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发表时间：
2015-01-01
影响因子：
2.2
通讯作者：
Li, Jinxiang
中科院分区：
文献类型：
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作者：
Li, Jiahe;Zhang, Jixiang;Li, Jinxiang
牛支原体 (M. bovis) 是一种与多种疾病综合征相关的病原体，这些疾病综合征会导致养牛业遭受重大经济损失。在这里，利用复制起点（oriC）区域开发了一种用于牛分枝杆菌的稳定复制质粒系统。此外，该质粒系统成功表达了异源蛋白β-半乳糖苷酶和FLAG标签融合的内源蛋白。这些发现证明这种基于 oriC 的载体适用于牛分枝杆菌的研究。 (C) 2014 Elsevier B.V. 保留所有权利。
Mycoplasma bovis (M. bovis) is a pathogen related to a variety of disease syndromes that result in significant economic losses in the cattle industry. Here, a stable replicative plasmid system is developed for use in M. bovis, utilizing an origin of replication (oriC) region. Additionally, the heterologous protein beta-galactosidase and a FLAG tag-fused endogenous protein were successfully expressed by this plasmid system. These findings provide evidence that this oriC-based vector is applicable for the study of M. bovis. (C) 2014 Elsevier B.V. All rights reserved.