Neurons with choline acetyltransferase immunoreactivity and mRNA are present in the human cerebral cortex
Neurons with choline acetyltransferase immunoreactivity and mRNA are present in the human cerebral cortex
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DOI：
10.1007/s004180050349
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发表时间：
1999-03-01
影响因子：
2.3
通讯作者：
Oda, Y
中科院分区：
文献类型：
--
作者：
Kasashima, S;Kawashima, A;Oda, Y
我们研究了5个尸检的人没有神经和精神疾病的大脑皮层免疫组化使用抗人重组胆碱乙酰转移酶（ChAT）多克隆抗体和原位杂交与S-35标记的人ChAT核糖核酸探针。免疫组织化学检测到阳性神经元，为中型或大型锥体神经元，主要分布在第III层和第V层，运动区和次级感觉区的神经元密度高于其他皮质区;第V层的免疫反应神经元在运动区分布更密集，第III层的免疫反应神经元分布在次级感觉区。在扣带回和海马旁浅层观察到阳性染色的非锥体神经元。初级感觉区未见免疫反应阳性神经元。原位杂交结果显示，在大脑皮质内有部分ChAT mRNA阳性神经元，主要分布在运动区的第V层和次级视区的第III层。这些结果表明，人类大脑皮层含有胆碱能神经元，并显示其分布的区域和层的变化。
We examined the cerebral cortex of five autopsied individuals without neurological and psychiatric diseases by immunohistochemistry using an anti-human recombinant choline acetyltransferase (ChAT) polyclonal antibody and in situ hybridization with S-35-labeled human ChAT riboprobes. The immunohistochemistry detected positive neurons which were medium-sized or large pyramidal neurons located predominantly in layers III and V. The density of such neurons was higher in the motor and secondary sensory areas than in other cortical areas; the immunoreactive neurons in layer V were more densely distributed in the motor area and those in layer III were distributed in the secondary sensory areas. Positively stained, non-pyramidal neurons were observed in the superficial layer of the cingulate gyrus and parahippocampus. No immunoreactive neurons were found in the primary sensory areas. The in situ hybridization detected some neurons with signals for ChAT mRNA in the cerebral cortex, most of which were distributed in layer V of the motor area and in layer III of the secondary visual area. These results indicate that the human cerebral cortex contains cholinergic neurons and displays regional and laminal variations in their distribution.