Coexpression of T4 and T8 on peripheral blood T cells demonstrated by two-color fluorescence flow cytometry.
Coexpression of T4 and T8 on peripheral blood T cells demonstrated by two-color fluorescence flow cytometry.
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DOI：
10.4049/jimmunol.134.4.2281
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发表时间：
1985-04
影响因子：
4.4
通讯作者：
M. Blue;J. Daley;H. Levine;S. Schlossman
中科院分区：
文献类型：
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作者：
M. Blue;J. Daley;H. Levine;S. Schlossman
使用双色荧光流式细胞术，我们能够检测到少量的T4+T8+细胞（约3%）的存在下，新鲜分离的外周血T细胞群来自正常的健康供体。T4和T8的共表达主要见于大胚样细胞，似乎与活化有关。用伴刀豆球蛋白A（Con A）刺激外周T细胞5天导致产生高达60%的T4+T8+细胞。共表达伴随着每细胞T8抗原位点数量的两倍增加。凝集素刺激培养物中的T4+T8+细胞表达高水平的活化抗原T9，T10和IL-2受体，但缺乏T6，一种在大多数II期胸腺细胞上发现的抗原。T4和T8的共表达似乎是一个短暂的过程，因为在缺乏凝集素的情况下延长T细胞的培养导致T4+T8+表型的丧失。我们的数据表明，外周血中的T细胞活化导致T4+T8+细胞群的产生，这与先前描述的胸腺和外周血细胞不同。因为T4和T8分子可以直接与MHC抗原相互作用，所以这些分子的共表达可能在免疫功能中具有重要作用。
Using two-color fluorescence flow cytometry, we were able to detect the presence of small numbers of T4+T8+ cells (about 3%) in freshly isolated peripheral T cell populations derived from normal healthy donors. Coexpression of T4 and T8 was predominantly found on large blastlike cells and appeared to be related to activation. Stimulation of peripheral T cells with concanavalin A (Con A) for 5 days resulted in the generation of up to 60% of T4+T8+ cells. Coexpression was accompanied by a twofold increase in the number of T8 antigenic sites per cell. The T4+T8+ cells in lectin-stimulated cultures expressed high levels of the activation antigens T9, T10, and the IL-2 receptor but lacked T6, an antigen found on a majority of stage II thymocytes. Coexpression of T4 and T8 appeared to be a transitory process, because prolonged culture of T cells in the absence of lectin resulted in the loss of the T4+T8+ phenotype. Our data suggest that T cell activation in peripheral blood results in the generation of a T4+T8+ cell population which is distinct from previously described thymic and peripheral blood cells. Because T4 and T8 molecules may interact directly with MHC antigens, coexpression of these molecules may have an important role in immune function.