Aggregation of Escherichia coli proteins during stationary phase depends on glucose and oxygen availability
Aggregation of Escherichia coli proteins during stationary phase depends on glucose and oxygen availability
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DOI：
10.1016/j.resmic.2008.09.008
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发表时间：
2008-11-01
影响因子：
2.6
通讯作者：
Laskowska, Ewa
中科院分区：
文献类型：
--
作者：
Kwiatkowska, Joanna;Matuszewska, Ewelina;Laskowska, Ewa
在自然环境中，细菌经常受到营养饥饿和其他压力的挑战。因此，细胞生长被阻止，细菌进入稳定期。在这份报告中，我们证明，在稳定期，大肠杆菌细胞积累聚集的错误折叠的蛋白质和复合物的Dps（饥饿诱导蛋白）与染色体DNA。我们发现，多组分蛋白质聚集体和不溶性Dps-DNA复合物的形成取决于生长条件，并受到培养基中氧气和葡萄糖的可用性的影响。需氧静止细胞生长在无缓冲培养基中补充葡萄糖含有不溶性Dps-DNA，而多组分蛋白质聚集体积累下葡萄糖饥饿。相反，在缺氧条件下，Dps-DNA复合物在无葡萄糖的情况下形成，而多组分蛋白质聚集体在葡萄糖的存在下出现。负责这种现象的机制仍有待阐明，然而，我们证明，在MOPS缓冲培养的不溶性Dps和蛋白质聚集体的水平下降。(C)2008年，Elsevier Masson SAS。All rights reserved.
In natural environments, bacteria are often challenged by nutrient starvation and other stresses. As a consequence, cell growth is arrested and bacteria enter stationary phase. In this report, we demonstrate that during stationary phase, Escherichia coli cells accumulate aggregates of misfolded proteins and complexes of Dps (starvation-induced protein) with chromosomal DNA. We found that the formation of multicomponent protein aggregates and insoluble Dps-DNA complexes depended on growth conditions and was influenced by the availability of oxygen and glucose in a medium. Aerobic stationary cells grown in unbuffered medium supplemented with glucose contained insoluble Dps-DNA, whereas multicomponent protein aggregates were accumulated under glucose starvation. On the contrary, under oxygen depletion, Dps-DNA complexes were formed in the absence of glucose, whereas multicomponent protein aggregates appeared in the presence of glucose. The mechanisms responsible for this phenomenon remain to be elucidated; however, we demonstrated that in MOPS-buffered cultures the level of insoluble Dps and protein aggregates was decreased. (C) 2008 Elsevier Masson SAS. All rights reserved.