THE YERSINIA-PSEUDOTUBERCULOSIS ADHESIN YADA MEDIATES INTIMATE BACTERIAL ATTACHMENT TO AND ENTRY INTO HEP-2 CELLS
THE YERSINIA-PSEUDOTUBERCULOSIS ADHESIN YADA MEDIATES INTIMATE BACTERIAL ATTACHMENT TO AND ENTRY INTO HEP-2 CELLS
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DOI：
10.1128/iai.61.9.3914-3921.1993
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发表时间：
1993-09-01
影响因子：
3.1
通讯作者：
FALKOW, S
中科院分区：
文献类型：
--
作者：
BLISKA, JB;COPASS, MC;FALKOW, S
我们描述了由耶尔森菌黏附素YadA介导的细菌-宿主细胞相互作用。将含有yadA、yopE、yopH或低钙反应调控位点突变的毒力质粒pIB1的衍生物引入假结核耶尔森菌YPIII菌株中，检测突变菌株粘附和进入HEp-2细胞的能力，并表达yopE和yopH的细胞毒活性。如先前所示，在没有Inv的情况下，YadA的表达是细菌附着和Yop活性所必需的（R. Rosqvist， A. Forsberg， M. Rimpilainen， T. Bergman, and H. Wolf-Watz, Mol. Microbiol. 4:657- 667,1990）。此外，当YadA表达而不表达YopE和YopH时，细菌能有效进入HEp-2细胞。这些结果表明，YopH介导假结核杆菌与HEp-2细胞的密切附着，YopH和YopE的协同作用抑制了该途径对细菌的吞噬摄取。HEp-2细胞粘附和单克隆抗体中和研究支持整合素作为宿主细胞受体在这种细菌附着和进入机制中的作用。
We characterized a bacterium-host cell interaction that is mediated by the Yersinia adhesin YadA. Derivatives of the virulence plasmid pIB1 harboring mutations in yadA, yopE, or yopH or in a low-calcium-response regulatory locus were introduced into a Yersinia pseudotuberculosis YPIII strain defective for Inv. The mutant strains were tested for the capacity to attach to and enter HEp-2 cells and express the cytotoxic activities of YopE and YopH. As previously shown, expression of YadA was necessary for bacterial attachment and Yop activity in the absence of Inv (R. Rosqvist, A. Forsberg, M. Rimpilainen, T. Bergman, and H. Wolf-Watz, Mol. Microbiol. 4:657-667, 1990). In addition, bacterial entry into HEp-2 cells occurred efficiently when YadA was expressed in the absence of YopE and YopH. These results demonstrated that YadA mediates intimate attachment of Y. pseudotuberculosis to HEp-2 cells and that phagocytic uptake of bacteria by this pathway is inhibited by the synergistic activities of YopH and YopE. A role for 13, integrins as host cell receptors for this bacterial attachment and entry mechanism was supported by HEp-2 cell adhesion and monoclonal antibody neutralization studies.