31P nuclear magnetic resonance and freeze-fracture electron microscopy studies on Escherichia coli. I. Cytoplasmic membrane and total phospholipids.
31P nuclear magnetic resonance and freeze-fracture electron microscopy studies on Escherichia coli. I. Cytoplasmic membrane and total phospholipids.
复制标题
大肠杆菌的 31P 核磁共振和冷冻断裂电子显微镜研究。
DOI：
10.1016/0005-2736(80)90222-9
复制
发表时间：
1980
期刊：
影响因子：
--
通讯作者：
B. de Kruijff
中科院分区：
文献类型：
--
作者：
E. Burnell;L. van Alphen;A. Verkleij;B. de Kruijff
1.在生长温度下，从大肠杆菌细胞中分离的总磷脂产生31 P-NMR谱，这表明存在层状，各向同性和六方相。这些阶段也检测冷冻断裂电子显微镜。特别地，各向同性相可以包含与层状相相关联的双相颗粒（可能是反相胶束）。2.从E.在37 ℃下生长的大肠杆菌细胞在25 ℃下主要是层状的，而在37和45 ℃下，表明存在一些几乎各向同性的磷脂运动。讨论了各向同性相对细胞质膜功能的可能意义。
1. At the growth temperature the total phospholipids isolated from Escherichia coli cells give rise to 31P-NMR spectra which indicate the existence of lamellar, isotropic and hexagonal phases. These phases are also detected by freeze fracture electron microscopy. In particular, the isotropic phase may contain lipidic particles (possibly inverted micelles) associated with the lamellar phase. 2. The cytoplasmic membrane isolated from E. coli cells grown at 37 degrees C is mainly lamellar at 25 degrees C, whereas at 37 and 45 degrees C the presence of some almost isotropic phospholipid motion is indicated. The possible significance of the isotropic phase for the functioning of the cytoplasmic membrane is discussed.