Fine mapping of the Ph-3 gene conferring resistance to late blight (Phytophthora infestans) in tomato
Fine mapping of the Ph-3 gene conferring resistance to late blight (Phytophthora infestans) in tomato
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DOI：
10.1007/s00122-013-2162-1
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发表时间：
2013-10-01
影响因子：
5.4
通讯作者：
Li, Junming
中科院分区：
文献类型：
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作者：
Zhang, Chunzhi;Liu, Lei;Li, Junming
晚疫病，由卵菌病原体致病疫霉（Phytophthora infestans（Mont.）de Bary是番茄和马铃薯作物的毁灭性病害。在过去的几十年中，许多马铃薯晚疫病抗性（R）基因已被鉴定。相比之下，对番茄的研究较少。将来自苦皮茄的Ph-3基因渗入栽培番茄中，并赋予其对晚疫病菌的广谱抗性。它以前被分配到9号染色体的长臂。本研究利用番茄CLN 2037 B（含Ph-3）与番茄S.番茄LA 4084。将Ph-3定位在两个标记Indel_3和P55之间的0.5cM间隔中。在番茄Heinz 1706参考基因组相应的74 kb区域中发现了8个推定基因。这些基因中有四个是具有APAF-1、R蛋白和CED-4结构域共享的典型核苷酸结合接头的抗性基因类似物（RGA）。每个RGA均与文特茄晚疫病抗性基因Rpi-vnt 1.1具有很高的同源性。瞬时基因沉默表明，该RGA家族的成员是Ph-3介导的番茄晚疫病抗性所必需的。此外，该RGA家族也存在于马铃薯基因组中，但RGA的数量高于番茄。
Late blight, caused by the oomycete pathogen Phytophthora infestans (Mont.) de Bary, is a devastating disease for tomato and potato crops. In the past decades, many late blight resistance (R) genes have been characterized in potato. In contrast, less work has been conducted on tomato. The Ph-3 gene from Solanum pimpinellifolium was introgressed into cultivated tomatoes and conferred broad-spectrum resistance to P. infestans. It was previously assigned to the long arm of chromosome 9. In this study, a high-resolution genetic map covering the Ph-3 locus was constructed using an F-2 population of a cross between Solanum lycopersicum CLN2037B (containing Ph-3) and S. lycopersicum LA4084. Ph-3 was mapped in a 0.5 cM interval between two markers, Indel_3 and P55. Eight putative genes were found in the corresponding 74 kb region of the tomato Heinz1706 reference genome. Four of these genes are resistance gene analogs (RGAs) with a typical nucleotide-binding adaptor shared by APAF-1, R proteins, and CED-4 domain. Each RGA showed high homology to the late blight R gene Rpi-vnt1.1 from Solanum venturii. Transient gene silencing indicated that a member of this RGA family is required for Ph-3-mediated resistance to late blight in tomato. Furthermore, this RGA family was also found in the potato genome, but the number of the RGAs was higher than in tomato.