DEVELOPMENT OF 1-CELL EMBRYOS FROM DIFFERENT STRAINS OF MICE IN CZB MEDIUM
DEVELOPMENT OF 1-CELL EMBRYOS FROM DIFFERENT STRAINS OF MICE IN CZB MEDIUM
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DOI：
10.1095/biolreprod42.3.432
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发表时间：
1990-03-01
影响因子：
3.6
通讯作者：
ZIOMEK, CA
中科院分区：
文献类型：
--
作者：
CHATOT, CL;LEWIS, JL;ZIOMEK, CA
将几种不同品系的小鼠单细胞胚胎在CZB培养基中培养至囊胚期。CZB培养基可培养CF1倍。培养第3天，在培养基中加入葡萄糖，使B6SJLF1/ j1细胞胚胎发育至囊胚期。胚胎发育所需的葡萄糖量在5.5至49.5 mM的浓度范围内进行滴定。除最高浓度外，所有测试的葡萄糖水平均支持桑葚胚和囊胚发育65-85%。试验了不同的CZB培养基对4株小鼠单细胞胚胎发育的支持能力。对于CF1和DBA/2J的胚胎(均为.倍。B6SJLF1/J)小鼠在体外发育表现出“2细胞阻滞”，在第3天添加葡萄糖的含谷氨酰胺的CZB培养基中，从1细胞阶段到桑葚胚和囊胚的最佳发育（79%和87%）。来自B6D2F1/J和CD1雌性小鼠的胚胎(均为。B6SJLF1/J雄性)在体外发育中不表现出“2细胞阻滞”，从培养开始，在含有谷氨酰胺和葡萄糖的CZB培养基中，桑葚胚期和囊胚期发育最佳（95%和50%）。
One-cell embryos from several different strains of mice have been cultured to the blastocyst stage in CZB medium. CZB medium can be used to culture CF1 .times. B6SJLF1/J 1-cell embryos to the blastocyst stage provided glucose is introduced into the medium on Day 3 of culture. The amount of glucose required for embryo development was titrated using a concentration range of 5.5 to 49.5 mM. With the exception of the highest concentration, all glucose levels tested supported 65-85% development to the morula and blastocyst stages. Variations of CZB medium were tested for their ability to support the development of 1-cell embryos from 4 strains of mice. For embryos from CF1 and DBA/2J (both .times. B6SJLF1/J) mice, which exhibit a "2-cell block" to development in vitro, CZB medium containing glutamine with the addition of glucose on Day 3 supported optimum development from the 1-cell stage to morula and blastocytsts (79% and 87%). For embryos from B6D2F1/J and CD1 female mice (both .times. B6SJLF1/J males), which do not exhibit a "2-cell block" to in vitro development, optimum development to morula and blastocyst stages (95% and 50%) was in CZB medium containing both glutamine and glucose from the start of culture.