Effect of Shenfu Injection on Slow Arrhythmia and Bone Marrow Mesenchymal Stem Cells
Effect of Shenfu Injection on Slow Arrhythmia and Bone Marrow Mesenchymal Stem Cells
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参附注射液对缓慢性心律失常及骨髓间充质干细胞的影响
DOI：
10.1166/jbt.2018.1841
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发表时间：
2018-07-01
影响因子：
0.1
通讯作者：
Wu, Hui
中科院分区：
文献类型：
--
作者：
Li, Rong;Zhao, XinJun;Wu, Hui
本研究旨在观察参附注射液对兔骨髓间充质干细胞（BMSCs）体外定向分化为心肌样细胞的影响。将18只新西兰大白兔随机分为对照组、参附注射液高剂量组和参附注射液低剂量组。每组细胞分为两个亚组（A和B），每组3只兔。诱导分化的效果通过细胞形态学、心肌细胞特异性蛋白（肌钙蛋白-T和α-肌动蛋白）以及干细胞因子（SCF）和粒细胞集落刺激因子（G-CSF）的检测来证实。培养28 d后，HE染色，光镜下细胞形态与心肌细胞相似。加入SFI和5-aza的组中肌钙蛋白-T的阳性强度高于仅加入5-aza的组（p < 0.05）;剂量高，而阳性强度高。同样的结果也发生在α-肌动蛋白上，但相关性很差，因为α-肌动蛋白对心肌没有特异性，样本量也很小。参附注射液高剂量组SCF水平高于对照组（p < 0.05）。与参附注射液高剂量组相比，参附注射液低剂量组和对照组的G-CSF水平明显降低（P < 0.01）。参附注射液对造血因子SCF和G-CSF有刺激作用，且与药物浓度呈正相关。结论：参附注射液联合5-aza可促进骨髓间充质干细胞向心肌样细胞的定向分化，提高心肌样细胞的阳性率。
This study is to determine the effect of Shenfu injection (SFI) on the directional differentiation of rabbit bone marrow mesenchymal stem cells (BMSCs) into cardiomyocyte-like cells in vitro. Eighteen New Zealand rabbits were randomly divided into the following three groups: control; high-dose SFI; and low-dose SFI. The cells in each group were divided into two sub-groups (A and B) with 3 rabbits. The effect of inducing differentiation was confirmed by cytomorphology, cardiomyocyte-specific protein (troponin-T and alpha-actin), and detection of stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF). After 28 days of culture, the cells were stained with HE, and the morphology of cultured BMSCs was similar to cardiomyocytes under light microscopy. Positive intensities of troponin-T in the groups with added SFI and 5-aza were higher than groups with added 5-aza only (p < 0.05); the dose was high, while positive intensity was high. The same results occurred with respect to alpha-actin, but the correlation was poor because alpha-actin is not specific to the myocardium and the sample size was small. The level of SCF in the high-dose SFI group was higher than the control group (p < 0.05). Compared with the high-dose SFI group, the levels of G-CSF were decreased in the low-dose SFI and control groups (p < 0.01). SFI had a stimulating effect on the hematopoietic factors (SCF and G-CSF), which was positively correlated with the drug concentration. In conclusion, SFI combined with 5-aza synergized the directional differentiation of BMSCs into cardiomyocytes and improved the positive rate of cardiomyocyte-like cells.