Morphine enhances myofilament CA(2+) sensitivity in intact guinea pig beating hearts.
Morphine enhances myofilament CA(2+) sensitivity in intact guinea pig beating hearts.
复制标题
吗啡可增强豚鼠完整跳动心脏中肌丝 CA(2) 的敏感性。
DOI：
10.1097/00000539-200103000-00009
复制
发表时间：
2001
影响因子：
5.7
通讯作者：
A. Namiki
中科院分区：
文献类型：
--
作者：
Y. Nakae;S. Fujita;A. Namiki
无标签
我们研究了在生理条件下，吗啡是否改变正常豚鼠心脏跳动中的细胞内钙离子浓度([Ca(2+)](I))、左室压(LVP)和肌丝钙敏感性，以及刺激Delta(1)、Delta(2)和kappa阿片是否与吗啡的直接心脏效应有关。根据385 nm和456 nm处的荧光信号测量跨壁左室相[Ca(2+)](I)。每次收缩过程中的Ca(2+)瞬变被定义为有效[Ca(2+)](I)。用含有吗啡的改良Krebs-Ringer液灌流心脏，同时记录LVP和有效[Ca(2+)](I)。吗啡(1微米)使有效[Ca(2+)](I)降低44+/-12 nM，但不降低2.5 mM[CaCl2](E)的发展LVP(P<；0.05)。吗啡(1微米)使发展的LVP曲线左移，这是有效[Ca(2+)](I)的函数(P<；0.05)。BNTX(1微米)可阻断吗啡(1微米)的作用，但不能阻断Nor-BNI(1微米)或NTB(0.1微米)对[Ca(2+)](I)的影响。在正常跳动的豚鼠心脏上，吗啡降低有效[Ca(2+)](I)，但不降低LVP，并在生理条件下增强肌丝对Ca(2+)的敏感性。Delta1阿片类刺激改变了吗啡对钙瞬变和肌丝钙敏感性的影响。
影响
吗啡以临床相关浓度改变豚鼠心脏肌丝Ca(2+)敏感性和Ca(2+)瞬变。
UNLABELLED
We investigated whether morphine alters intracellular Ca(2+) concentration ([Ca(2+)](i)), left ventricular pressure (LVP), and myofilament Ca(2+) sensitivity under physiologic conditions in intact guinea pig beating hearts and whether delta(1), delta(2), and kappa opioid stimulations are related to the direct cardiac effects of morphine. Transmural LV phasic [Ca(2+)](i) was measured from fluorescence signals at 385 nm and 456 nm. The Ca(2+) transients during each contraction were defined as available [Ca(2+)](i). The hearts were perfused with modified Krebs-Ringer solution containing morphine in the absence and presence of delta(1) (BNTX), delta(2) (NTB), and kappa (nor-BNI) antagonists, while developed LVP and available [Ca(2+)](i) were recorded. Morphine (1 microM) decreased available [Ca(2+)](i) by 44 +/- 12 nM without decreasing developed LVP at 2.5 mM of [CaCl(2)](e) (P < 0.05). Morphine (1 microM) caused a leftward shift in the curve of developed LVP as a function of available [Ca(2+)](i) (P < 0.05). BNTX (1 microM), but not nor-BNI (1 microM) or NTB (0.1 microM) blocked morphine (1 microM) effects to decrease available [Ca(2+)](i). Morphine decreases available [Ca(2+)](i) but not LVP, and it enhances myofilament Ca(2+) sensitivity under physiologic conditions at clinical concentrations in intact isolated beating guinea pig hearts. The delta(1) opioid stimulation modifies the effects of morphine on Ca(2+) transients and myofilament Ca(2+) sensitivity.
IMPLICATIONS
Morphine modifies myofilament Ca(2+) sensitivity and Ca(2+) transients in guinea pig hearts at concentrations that are clinically relevant.
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使用 Indo-1 荧光测定法对整个灌注大鼠心脏中的胞质 [Ca2] 进行定量。
影响因子：
3.4
作者：
Brandes,R;Figueredo,VM;Camacho,SA;Baker,AJ;Weiner,MW
通讯作者：
Weiner,MW
DOI：
10.1152/ajpheart.1999.277.6.h2321
发表时间：
1999-12
期刊：
American journal of physiology. Heart and circulatory physiology
影响因子：
--
作者：
D. Stowe;S. Fujita;J. An;R. A. Paulsen;S. G. Varadarajan;S. Smart
通讯作者：
D. Stowe;S. Fujita;J. An;R. A. Paulsen;S. G. Varadarajan;S. Smart
补偿缺氧灌注大鼠心脏荧光测定中组织光吸收的变化。
DOI：
10.1152/ajpheart.1994.266.6.h2554
发表时间：
1994
期刊：
The American journal of physiology
影响因子：
--
作者：
Brandes,R;Figueredo,VM;Camacho,SA;Weiner,MW
通讯作者：
Weiner,MW
改变肌原纤维对 Ca 的反应作为正性肌力机制。
影响因子：
37.8
作者：
Blinks,JR;Endoh,M
通讯作者：
Endoh,M