Principle, Validity, and Reliability of Scanning Laser Doppler Flowmetry
Principle, Validity, and Reliability of Scanning Laser Doppler Flowmetry
复制标题
DOI：
10.1097/00061198-199604000-00004
复制
发表时间：
1996-04
影响因子：
2
通讯作者：
G. Michelson;B. Schmauss;M. Langhans;J. Harazny;M. Groh
中科院分区：
文献类型：
--
作者：
G. Michelson;B. Schmauss;M. Langhans;J. Harazny;M. Groh
目的本研究的目的是提供扫描激光多普勒血流仪（SLDF）的可靠性和有效性，该仪器可对视网膜和视神经乳头的灌注血管进行高清晰度地形图，同时评价血流。方法SLDF是利用光学多普勒效应检测血流的。数据采集和评估系统是一种改进的激光扫描装置;激光源的波长为670 nm，功率为100 μW（Heidelberg Engineering，HRF）。通过对10只眼进行5次独立测量，评价SLDF的可靠性。通过两个实验验证了该方法的有效性。首先，在一个实验装置中，SLDF的能力，以绝对单位测量移动平面的速度进行了估计。第二，比较测量视网膜血流在16个正常的眼睛和33个青光眼的眼睛与SLDF和市售的单点激光多普勒流量计（Oculix）。结果SLDF具有较高的可靠性。流量、容积和流速的信度系数分别为0.82、0.81和0.83。通过SLDF和相应视网膜点的单点激光多普勒流量计对视网膜血流的比较测量显示，流量（r = 0.83，p < 0.0001）、体积（r = 0.51，p < 0.0001）和速度（r = 0.59，p < 0.0001）之间存在线性和显著关系。在实验装置中，SLDF能够以绝对单位定量测量速度。结论SLDF通过光学多普勒频移的二维映射和毛细血管血流的可重复评估，能够以高分辨率显示视网膜乳头和视神经乳头的灌注血管。
PurposeThe objective of this study is to present the reliability and validity of scanning laser Doppler flowmetry (SLDF) performing a high-definition topography of perfused vessels of the retina and the optic nerve head with simultaneous evaluation of blood flow. MethodsThe examination of blood flow by SLDF is based on the optical Doppler effect. The data aquisition and evaluation system is a modified laser scanning device; the wavelength of the laser source is 670 nm, with a power of 100 μW (Heidelberg Engineering, HRF). The reliability of SLDF was estimated by performing five seperate measurements in 10 eyes on 5 days. The validity of the method was tested by two experiments. First, in an experimental set-up, the capability of SLDF to measure the velocity of a moving plane in absolute units was estimated. Second, comparative measurements were performed of retinal blood flow in 16 normal eyes and in 33 glaucomatous eyes with SLDF and a commercially available single-point laser Doppler flowmeter (Oculix). ResultsWe found SLDF to produce a high reliability. The reliability coefficients rx of flow, volume, and velocity were 0.82, 0.81, and 0.83, respectively. Comparative measurements of the retinal blood flow by SLDF and a single-point laser Doppler flowmeter of corresponding retinal points showed a linear and significant relationship between flow (r = 0.83, p < 0.0001), volume (r = 0.51, p < 0.0001), and velocity (r = 0.59, p < 0.0001). In the experimental set-up, SLDF was able to quantitatively measure velocity in absolute units. ConclusionsSLDF enables the visualization of perfused vessels of the jux-tapapillary retina and the optic nerve head in high resolution by two-dimensional mapping of the optical Doppler shift and a reproducible evaluation of capillary blood flow.