Patterns of innervation of sympathetic vascular neurons by peptide-containing primary sensory fibers.
Patterns of innervation of sympathetic vascular neurons by peptide-containing primary sensory fibers.
复制标题
含肽初级感觉纤维对交感血管神经元的神经支配模式。
DOI：
10.1016/s0006-8993(99)01313-x
复制
发表时间：
1999
期刊：
影响因子：
2.9
通讯作者：
Kreulen,DL
中科院分区：
文献类型：
--
作者：
Zheng,ZL;Travagli,RA;Kreulen,DL
本研究的目的是确定豚鼠肠系膜下神经节（IMG）血管神经元的初级感觉神经支配是否存在特定的组织。在体外肠系膜下动脉（IMA）或肠系膜下静脉（IMV）腔内放置逆行示踪剂，以识别神经节神经元为动脉、静脉或未标记的神经元。比较辣椒素治疗前后含降钙素基因相关肽（CGRP）、神经元一氧化氮合酶（NOS）和P物质免疫反应性（SP-IR）的初级感觉神经纤维分布。在对照动物中，免疫反应的密度随递质和受支配的神经元类型而变化。辣椒素处理降低了三种物质的免疫反应密度。神经纤维免疫反应性降低的程度随递质和神经元类型的不同而不同。未标记神经元周围CGRP和SP免疫反应纤维密度最大；78%的CGRP纤维为初级感觉纤维，所有SP纤维为初级感觉纤维。动脉神经元周围44%的CGRP纤维为初级感觉来源，静脉周围68%为初级感觉来源。静脉神经元周围NOS阳性神经分布较动脉神经元周围密集，且全部（97%）被辣椒素完全消除，表明其完全是初级感觉来源。我们得出结论，支配IMG的初级感觉纤维在动脉和静脉神经元上的分布是不同的，并且每种感觉神经递质的分布模式都是有特点的。
The purpose of this study was to determine whether there is a specific organization of the primary sensory innervation on to identified vascular neurons in the inferior mesenteric ganglion (IMG) in guinea-pig. Retrograde tracers were placed intraluminally in inferior mesenteric artery (IMA) or inferior mesenteric vein (IMV) in vitro to identify ganglionic neurons as arterial, venous or unlabeled neurons. The distribution of primary sensory nerve fibers containing calcitonin gene-related peptide (CGRP), neuronal nitric oxide synthase (NOS) and substance P immunoreactivity (SP-IR) was compared before and after treatment with capsaicin. In control animals the density of immunoreactivity varied both with the transmitter and the type of neuron innervated. The density of immunoreactivity for all the three substances was reduced by capsaicin treatment. The degree of reduction of immunoreactivity in the fibers varied with the transmitter and the type of neuron. The density of CGRP and SP immunoreactive fibers was greatest around unlabeled neurons; 78% of the CGRP fibers were of primary sensory origin and all of the SP fibers were primary sensory. Around arterial neurons 44% of the CGRP fibers were of primary sensory origin and around venous 68% were primary sensory. NOS positive innervation around venous neurons was denser than around arterial neurons and all of it was completely (97%) eliminated by capsaicin, indicating that it was solely of primary sensory origin. We conclude that the primary sensory fibers innervating the IMG are differentially distributed to arterial and venous neurons and that the pattern of distribution is characteristic for each sensory neurotransmitter.