THE S INDEX IN THE RETENTION EQUATION IN REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
THE S INDEX IN THE RETENTION EQUATION IN REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
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DOI：
10.1016/0021-9673(92)85343-r
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发表时间：
1992-06-19
期刊：
影响因子：
--
通讯作者：
LU, PC
中科院分区：
文献类型：
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作者：
CHEN, N;ZHANG, YK;LU, PC
研究了反相高效液相色谱（RP-HPLC）保留值经验方程logk ' = logk（w）' - S-phi，考察了参数S的性质。在RP-HPLC中，系统地检查了S指数，S指数定义为log k'相对于有机改性剂体积分数（phi）的斜率，其为键合相密度、柱类型和温度的函数。对于一个特定的溶质的S指数被观察到几乎是恒定的，即使当使用不同的C18填充材料的柱系统。log k'对洗脱液组成的依赖性被发现表示为一个给定的溶质为各种不同的固定相的平行线。尽管C18柱长期使用，对于给定的溶质，S指数保持恒定。结果表明，S指数主要取决于溶质与移动的相之间的相互作用。对于非离子溶质，观察到随着柱温的升高而降低。讨论了影响S指数测量的其他因素。
The empirical retention equation log k' = log k(w)' - S-phi in reversed-phase high-performance liquid chromatography (RP-HPLC) was investigated to evaluate the properties of the parameter S. The S index, which is defined as the slope of log k' versus volume fraction of the organic modifier (phi) was systematically examined as a function of bonded phase density, column type and temperature in RP-HPLC. The S index for a particular solute was observed to be nearly constant even when column systems with different C18 packing materials are used. The dependence of log k' on eluent composition was found to be represented by parallel lines for a given solute for a variety of different stationary phases. The S index remains constant for a given solute despite the prolonged use of C18 column. The results showed that the S index is determined mainly by the interaction between the solute and the mobile phase. It was observed to decrease with the increasing column temperature for non-ionic solutes. Other factors influencing the measurement of the S index are discussed.