Evaluating the solid retention time of bacteria in flocculent and granular sludge.
Evaluating the solid retention time of bacteria in flocculent and granular sludge.
复制标题
DOI：
10.1016/j.watres.2012.06.027
复制
发表时间：
2012-10
期刊：
影响因子：
12.8
通讯作者：
M. Winkler;R. Kleerebezem;W. Khunjar;B. de Bruin;M. V. van Loosdrecht
中科院分区：
文献类型：
--
作者：
M. Winkler;R. Kleerebezem;W. Khunjar;B. de Bruin;M. V. van Loosdrecht
测定了不同细菌在絮状和颗粒污泥中的特定固体保留时间。从反应器和流出污泥中收集样品，并通过定量聚合酶链反应（qPCR）评估特定细菌群落相对于总细菌群落的数量。确定反应器污泥和废弃污泥中特定细菌群的相对存在比例，以观察是否发生优先冲洗。从数据中还可以估计不同微生物群的固体保留时间。使用此工具，我们能够证明颗粒外部群体的 SRT 略低于内部群体的 SRT。在絮状系统中没有发现古细菌，但在颗粒系统中存在少量。进一步观察到，原生动物以系统内的细菌群落为食，这表明它们有可能缩短细菌的特定 SRT。
The specific solid retention time for different bacteria within flocculent and granular sludge was determined. Samples were collected from reactor and effluent sludge and the number of a specific bacterial group was evaluated in respect to the total bacterial community with quantitative polymerase chain reaction (qPCR). The ratio of the relative presence of a specific bacterial group in the reactor sludge and wasted sludge was established to observe if preferential wash-out occurred. From the data also the solid retention time for different microbial groups can be estimated. Using this tool, we were able to show that the SRT of populations found on the exterior of granules is slightly lower than the SRT for population in the interior. Archaea were not found in the flocculent system but were present in small amounts within the granular system. It was further observed that protozoa were grazing on the bacterial community within the system indicating that they have the potential to shorten the specific SRT of bacteria.