RECEPTOR-MEDIATED UTERINE EFFECTS OF VASOPRESSIN AND OXYTOCIN IN NONPREGNANT WOMEN
RECEPTOR-MEDIATED UTERINE EFFECTS OF VASOPRESSIN AND OXYTOCIN IN NONPREGNANT WOMEN
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DOI：
10.1111/j.1471-0528.1995.tb10880.x
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发表时间：
1995-11-01
期刊：
影响因子：
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通讯作者：
MAGGI, M
中科院分区：
文献类型：
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作者：
BOSSMAR, T;AKERLUND, M;MAGGI, M
目的研究子宫加压素和催产素在非妊娠妇女子宫肌层的作用，以及特定的子宫受体是否参与这些作用。静脉推注10pmol/kg体重的加压素和催产素。重复采血测定血浆中加压素和催产素浓度。加压素和催产素对离体子宫肌层作用的记录。子宫肌层血管加压素VIA和催产素受体浓度的估计。测量血浆雌二醇和孕酮。主要结果测量加压素和催产素引起的体内记录曲线下面积增加超过10分钟，以及体外剂量反应的EC(50)浓度。结果在体内，加压素的活性平均是催产素的4倍。后叶加压素在月经前期的作用比单独使用雌激素的妇女更明显(增殖期-过度增殖；P=0.02)，而且倾向于比黄体期更明显(P=0.07)。未观察到催产素反应随激素状态的显著变化。加压素的EC(50)浓度比催产素低20多倍。加压素通过受体的中位数为208(139-343)fmol/mg蛋白，催产素受体的中位数为49(38-87)fmol/mg蛋白。加压素受体浓度与体内效应不相关，而催产素受体浓度与体内效应显著相关(P=0.02)。结论加压素在非妊娠妇女中的高效价，特别是在月经前期，有力地支持了加压素在原发性痛经子宫过度活动中的病因学意义。催产素在这种情况下似乎不那么重要，因为它的效力要小得多，而且在经前没有增加效果。加压素似乎通过子宫中的受体位置同时影响催产素和加压素，而催产素则特异性地作用于自己的受体。
Objective To study in nonpregnant women myometrial actions of vasopressin and oxytocin and the involvement in these effects of specific uterine receptors.Subjects Twenty-eight women undergoing hysterectomy for benign gynaecological disorders.Interventions Intrauterine pressure recordings. Intravenous bolus injections of 10 pmol/kg body weight of vasopressin and oxytocin. Repeated blood sampling for measurement of vasopressin and oxytocin concentrations in plasma. Recording of effects of vasopressin and oxytocin on isolated myometrium. Estimation of myometrial concentrations of vasopressin Via and oxytocin receptors. Measurement of plasma oestradiol and progesterone.Main outcome measures Vasopressin- and oxytocin-induced increases of the area under the in vivo recording curve over 10 minutes and EC(50) concentrations of dose-responses in vitro. Concentrations of vasopressin Via and oxytocin receptors.Results Vasopressin was on average four times more potent than oxytocin in vivo. The effect of vasopressin premenstrually was more pronounced than in women under oestrogen influence only (proliferative phase-hyperproliferation; P = 0.02), and tended to be more marked than in those in the luteal phase (P = 0.07). No significant variation in oxytocin response with the hormonal state was observed. EC(50) concentrations of vasopressin were more than 20 times lower than those of oxytocin. The median concentration of the vasopressin Via receptor was 208 (range 139-343) fmol/mg protein and that of the oxytocin receptor 49 (38-87) fmol/mg protein. Vasopressin receptor concentrations and in vivo effects of this peptide did not correlate, whereas for those of oxytocin a significant correlation was observed (P = 0.02).Conclusion The high potency of vasopressin in nonpregnant women, particularly premenstrually, firmly supports an aetiological importance of this peptide in the uterine hyperactivity of primary dysmenorrhoea. Oxytocin seems to be less important in this condition in view of its much smaller potency and the absence of increase in effect premenstrually. Vasopressin appears to influence both the oxytocin and the vasopressin Via receptor sites in the uterus, whereas oxytocin acts specifically on its own receptor.