SERS Detection of Breast Cancer-Derived Exosomes Using a Nanostructured Pt-Black Template
SERS Detection of Breast Cancer-Derived Exosomes Using a Nanostructured Pt-Black Template
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DOI：
10.1002/adsr.202200039
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发表时间：
2023-04-01
期刊：
影响因子：
--
通讯作者：
Thompson, Alex J.
中科院分区：
文献类型：
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作者：
Li, Xinyue;Keshavarz, Meysam;Thompson, Alex J.
目前，还没有一种既无创又高精度的癌症治疗方法。能够在早期阶段诊断癌症的新测试将有助于促进这种改进的治疗方法，最近的许多研究都集中在为此目的开发液体活检测试。外泌体是细胞分泌的细胞外囊泡，作为一种通讯手段，可以简单地从血液样本中收集到。目前的研究表明，表面增强拉曼光谱（SERS）在区分癌细胞和健康细胞方面具有潜力。在这里，一个定制的铂黑（Pt-black） SERS模板被开发-通过一种具有成本效益的电镀制造方法-检测故障（癌）外泌体。结果表明，Pt-black SERS衬底具有稳定一致的光谱，为可靠的诊断模板提供了高重现性。更重要的是，使用Pt-black SERS模板可以区分癌症来源的外泌体（从4T1细胞-一种三阴性乳腺癌细胞系中提取）和健康成纤维细胞的外泌体，敏感性为83.3%，特异性为95.8%。本研究确立了Pt-black模板检测癌性外泌体的潜力，为SERS在癌症诊断中的临床应用研究奠定了坚实的基础。据报道，一种定制的铂黑模板用于表面增强拉曼光谱及其用于癌外泌体的快速鉴定。作为人类三阴性乳腺癌的模型，使用来自4T1细胞的外泌体。使用开发的诊断模板在区分癌变外泌体和健康外泌体方面显示出83.3%的敏感性和95.8%的特异性。图像
At present, there are no cancer treatments that are both non-invasive and highly accurate. New tests that can diagnose cancer at an early stage would help to facilitate such improved therapies, and many recent studies have focused on the development of liquid biopsy tests for this purpose. Exosomes are extracellular vesicles secreted by cells as a means of communication that can be simply collected from blood samples. Current studies have shown the potential of surface-enhanced Raman spectroscopy (SERS) in differentiating cancerous cells from healthy cells. Herein, a bespoke platinum-black (Pt-black) SERS template is developed-via a cost-effective fabrication method of electroplating-to detect malfunctioned (cancerous) exosomes. The results demonstrate that the Pt-black SERS substrate exhibits stable and consistent spectra, which produces the high reproducibility required for a reliable diagnostic template. More importantly, using the Pt-black SERS template allows for the differentiation of cancer-derived exosomes (extracted from 4T1 cells-a triple-negative breast cancer cell line) and exosomes from healthy fibroblast cells with an 83.3% sensitivity and a 95.8% specificity. This study establishes the potential of the Pt-black template in detecting cancerous exosomes and lays a solid foundation for future studies in the clinical application of SERS in cancer diagnosis.The development of a bespoke platinum-black template for surface-enhanced Raman spectroscopy and its use for the rapid identification of cancerous exosomes is reported. As a model for human triple-negative breast cancer, exosomes derived from 4T1 cells are used. The results using the developed diagnostic template demonstrate 83.3% sensitivity and 95.8% specificity in differentiating cancerous from healthy exosomes. image