SARS-CoV-2 Antibody Responses Do Not Predict COVID-19 Disease Severity
SARS-CoV-2 Antibody Responses Do Not Predict COVID-19 Disease Severity
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DOI：
10.1093/ajcp/aqaa123
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发表时间：
2020-10-01
影响因子：
3.5
通讯作者：
Muthukumar, Alagarraju
中科院分区：
文献类型：
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作者：
Phipps, William S.;SoRelle, Jeffrey A.;Muthukumar, Alagarraju
目标：初步报告表明，一些基于血清学的严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 检测具有足够的性能。然而，还需要进行更多研究来促进结果的解释，包括抗体水平如何影响免疫力和疾病进程。 方法：总共对 967 名受试者进行了 SARS-CoV-2 反应性 IgG 抗体检测，其中包括 172 名 SARS-CoV-2 疑似病例、来自健康捐献者的 656 份血浆样本、来自风湿性疾病患者的 49 份血清以及来自基于聚合酶链反应 (PCR) 的呼吸道病毒检测呈阳性的个体的 90 份样本。采用蛋白质组芯片法对 SARS-CoV-2 PCR 阳性病例的亚组进行 IgM 抗体检测。结果：所有特异性和交叉反应性标本的 SARS-CoV-2 IgG 抗体均为阴性（0/795，0%）。在症状出现超过 14 天后确认的样本中，83% 的 IgG 与 PCR 呈阳性一致，对于严重免疫抑制的病例，敏感性低于 100%。症状出现后 3 天以内的病例中，病毒特异性 IgM 呈阳性的比例较高。在 IgG 和 IgM 水平方面，未观察到轻度和重度病程之间存在关联。结论：所研究的 SARS-CoV-2 IgG 检测具有 100% 的特异性，并且没有不良交叉反应性。 IgG 和 IgM 抗体的测量并不能预测我们患者群体的疾病严重程度。
Objectives: Initial reports indicate adequate performance of some serology-based severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) assays. However, additional studies are required to facilitate interpretation of results, including how antibody levels impact immunity and disease course.Methods: A total of 967 subjects were tested for IgG antibodies reactive to SARS-CoV-2, including 172 suspected cases of SARS-CoV-2, 656 plasma samples from healthy donors, 49 sera from patients with rheumatic disease, and 90 specimens from individuals positive for polymerase chain reaction (PCR)-based respiratory viral panel. A subgroup of SARS-CoV-2 PCR-positive cases was tested for IgM antibodies by proteome array method.Results: All specificity and cross-reactivity specimens were negative for SARS-CoV-2 IgG antibodies (0/795, 0%). Positive agreement of IgG with PCR was 83% of samples confirmed to be more than 14 days from symptom onset, with less than 100% sensitivity attributable to a case with severe immunosuppression. Virus-specific IgM was positive in a higher proportion of cases less than 3 days from symptom onset. No association was observed between mild and severe disease course with respect to IgG and IgM levels.Conclusions: The studied SARS-CoV-2 IgG assay had 100% specificity and no adverse cross-reactivity. Measures of IgG and IgM antibodies did not predict disease severity in our patient population.