Raman spectroscopic signature of blood and its potential application to forensic body fluid identification
Raman spectroscopic signature of blood and its potential application to forensic body fluid identification
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DOI：
10.1007/s00216-009-3207-9
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发表时间：
2010-01-01
影响因子：
4.3
通讯作者：
Lednev, Igor K.
中科院分区：
文献类型：
--
作者：
Virkler, Kelly;Lednev, Igor K.
近红外（NIR）拉曼光谱法用于测量来自多个供体的干燥人血液样品的光谱。在本文中解决的两个主要问题涉及的影响，样品的异质性和潜在的拉曼光谱的变化，可能会出现在不同的献血者之间。从干样品上的多个点获得的光谱的高级统计分析表明，干血液是化学不均匀的，并且其拉曼光谱可以很好地呈现为荧光背景和分别由血红蛋白和纤维蛋白主导的两个拉曼光谱组分的线性组合。每个样品的拉曼光谱包含相同的主峰，但血红蛋白和纤维蛋白组分的相对贡献随供体而变化。因此，没有一个单一的光谱可以以定量的方式充分代表干血的实验拉曼光谱，而是血红蛋白和纤维蛋白光谱成分的组合可以被认为是血液的光谱特征。这种概念验证方法显示了拉曼光谱法用于法医分析以识别未知物质（如血液）的潜力。
Near-infrared (NIR) Raman spectroscopy was used to measure spectra of dried human blood samples from multiple donors. Two major questions addressed in this paper involve the influence of sample heterogeneity and potential Raman spectral variations that could arise between different donors of blood. Advanced statistical analysis of spectra obtained from multiple spots on dry samples showed that dry blood is chemically heterogeneous, and its Raman spectra could be presented very well as a linear combination of a fluorescent background and two Raman spectroscopic components that are dominated by hemoglobin and fibrin, respectively. Each sample Raman spectrum contains the same major peaks, but the relative contribution of the hemoglobin and fibrin components varies with the donor. Therefore, no single spectrum could adequately represent an experimental Raman spectrum of dry blood in a quantitative way, but rather the combination of hemoglobin and fibrin spectral components could be considered to be a spectroscopic signature for blood. This proof-of-concept approach shows the potential for Raman spectroscopy to be used in forensic analysis to identify an unknown substance such as blood.