The use of rats and mice as animal models in ex vivo bone growth and development studies.
The use of rats and mice as animal models in ex vivo bone growth and development studies.
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DOI：
10.1302/2046-3758.512.bjr-2016-0102.r2
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发表时间：
2016-12
影响因子：
4.6
通讯作者：
Loqman MY
中科院分区：
文献类型：
--
作者：
Abubakar AA;Noordin MM;Azmi TI;Kaka U;Loqman MY
体内动物实验一直是生物学和生物医学研究的基石之一，特别是在临床医学和制药领域。传统的体内模型系统总是与高生产成本和严格的伦理考虑相关。这些限制导致了离体模型系统的发展，其部分或完全克服了体内模型系统中面临的一些约束。离体啮齿动物骨培养系统已被用于阐明骨骼生理学和病理生理学的理解超过90年。本文对啮齿类动物骨培养系统的历史演变进行了简要的总结，并强调了该模型的优点和局限性。它包括使用大鼠和小鼠进行离体骨研究的频率、离体骨生长的营养需求以及新兴的发展和技术。这些信息的汇编可以帮助再生医学和骨组织工程领域的研究人员更好地了解骨骼的生长和发育，以便在一般临床医学中应用。引用这篇文章：A。A. Abubakar，M. M. Noordin，T. I.阿兹米大学Kaka，M. Y.洛克曼在离体骨生长和发育研究中使用大鼠和小鼠作为动物模型。骨关节研究2016;5：610-618。DOI：10.1302/2046-3758.512.BJR-2016-0102.R2。
In vivo animal experimentation has been one of the cornerstones of biological and biomedical research, particularly in the field of clinical medicine and pharmaceuticals. The conventional in vivo model system is invariably associated with high production costs and strict ethical considerations. These limitations led to the evolution of an ex vivo model system which partially or completely surmounted some of the constraints faced in an in vivo model system. The ex vivo rodent bone culture system has been used to elucidate the understanding of skeletal physiology and pathophysiology for more than 90 years. This review attempts to provide a brief summary of the historical evolution of the rodent bone culture system with emphasis on the strengths and limitations of the model. It encompasses the frequency of use of rats and mice for ex vivo bone studies, nutritional requirements in ex vivo bone growth and emerging developments and technologies. This compilation of information could assist researchers in the field of regenerative medicine and bone tissue engineering towards a better understanding of skeletal growth and development for application in general clinical medicine. Cite this article: A. A. Abubakar, M. M. Noordin, T. I. Azmi, U. Kaka, M. Y. Loqman. The use of rats and mice as animal models in ex vivo bone growth and development studies. Bone Joint Res 2016;5:610–618. DOI: 10.1302/2046-3758.512.BJR-2016-0102.R2.