Evidence for occurrence of an organophosphate-resistant type of acetylcholinesterase in strains of sea lice (Lepeophtheirus salmonis Kroyer)
Evidence for occurrence of an organophosphate-resistant type of acetylcholinesterase in strains of sea lice (Lepeophtheirus salmonis Kroyer)
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DOI：
10.1002/ps.932
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发表时间：
2004-12-01
影响因子：
4.1
通讯作者：
Horsberg, TE
中科院分区：
文献类型：
--
作者：
Fallang, A;Ramsay, JM;Horsberg, TE
乙酰胆碱酯酶(AChE)是一个主要的农药家族--有机磷的靶标，在20世纪90年代初被广泛用于防治养殖鲑鱼的海虱。自20世纪90年代中期以来，由于抗药性的发展，有机磷、敌敌畏和氮甲硫磷受到严重影响。AChE对有机磷化疗药物不敏感已被认为是许多节肢动物的主要抗性机制，在本研究中，从挪威和加拿大的几个养鱼区分离的甲壳类动物鲑鱼Lepephtheirus salmonis Kroyer中证实了靶点抗性。双分子速率分析表明，存在两种对硫胺磷具有不同敏感性的AChE酶，一种是快速失活的，另一种是非常缓慢失活的。据我们所知，这是甲壳类第三类节肢动物对有机磷的靶点抗药性的第一次报告。(C)2004年化学工业学会
Acetylcholinesterase (AChE) is the target of a major pesticide family, the organophosphates, which were extensively used as control agents of sea lice on farmed salmonids in the early 1990s. From the mid-1990s the organophosphates dichlorvos and azamethiphos were seriously compromised by the development of resistance. AChE insensitive to organophosphate chemotherapeutants has been identified as a major resistance mechanism in numerous arthropod species, and in this study, target-site resistance was confirmed in the crustacean Lepeophtheirus salmonis Kroyer isolated from several fish-farming areas in Norway and Canada. A bimolecular rate assay demonstrated the presence of two AChE enzymes with different sensitivities towards azamethiphos, one that was rapidly inactivated and one that was very slowly inactivated. To our knowledge this is the first report of target-site resistance towards organophosphates in a third class of arthropods, the Crustacea. (C) 2004 Society of Chemical Industry