High energy gas cluster ions for organic and biological analysis by time-of-flight secondary ion mass spectrometry
High energy gas cluster ions for organic and biological analysis by time-of-flight secondary ion mass spectrometry
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DOI：
10.1016/j.ijms.2014.05.015
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发表时间：
2015-02-01
影响因子：
1.8
通讯作者：
Fletcher, John S.
中科院分区：
文献类型：
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作者：
Angerer, Tina B.;Blenkinsopp, Paul;Fletcher, John S.
气体团簇离子束（GCIBs）用于西姆斯分析，以研究有机材料和生物样品，如细胞和组织，有相当大的兴奋。这些离子束通常在初级离子中包含数千个氩原子，主要用于蚀刻有机材料以从表面去除损伤，从而允许进行分子深度剖析实验。离子束的能量通常为2-20 keV。关于使用GCIB作为分析束的研究报道相对较少，这是由于与束的快速脉冲和聚焦相关的困难沿着有时低的电离效率。在这项研究中，我们报告使用一个新的更高的能量（40键）GCIB在连续模式下运行。当与较低能量的Irganox 1010薄膜上的深度分布相比时，溅射产率增加，而碎片、损伤累积和电离效率保持不变。脑组织实验显示，与40 keV的C-60（+）和20个关键撞击能量的Ar-4000（+）相比，信号水平增加，尤其是对于较高质量的二次离子（m/z 500+）。较高能量的使用促进初级离子束的更好聚焦，如这里在人类头发样本上所展示的，其中我们实现了
There is considerable excitement surrounding the application of gas cluster ion beams (GCIBs) for SIMS analysis in order to study organic materials and biological samples such as cells and tissues. These ion beams, that often comprise several thousand argon atoms in the primary ion, have been used mainly for the etching of organic materials to remove damage from the surface allowing molecular depth profiling experiments to be performed. The energy of the ion beam is normally 2-20 keV. There have been relatively few studies reported on the use of GCIB as analysis beams, due to difficulties related to fast pulsing and focusing of the beam along with the sometimes low ionisation efficiency. In this study, we report on the use of a new higher energy (40 key) GCIB operated in a continuous mode. When compared to lower energies depth profiles on thin films of Irganox 1010 show an increase in sputter yield while fragmentation, damage accumulation and ionisation efficiency remains unchanged. Experiments on brain tissues show increased signal levels especially for higher mass secondary ions (m/z 500+) in comparison to C-60(+) at 40 keV and Ar-4000(+) at 20 key impact energy. The use of higher energies facilitates better focusing of the primary ion beam as demonstrated here on a human hair sample where we achieve a spatial resolution of