Nuclear envelope breakdown in starfish oocytes proceeds by partial NPC disassembly followed by a rapidly spreading fenestration of nuclear membranes.
Nuclear envelope breakdown in starfish oocytes proceeds by partial NPC disassembly followed by a rapidly spreading fenestration of nuclear membranes.
复制标题
海星卵母细胞中的核包膜分解是部分NPC拆卸进行的，然后迅速扩散核膜。
DOI：
10.1083/jcb.200211076
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发表时间：
2003-03-31
影响因子：
7.8
通讯作者：
Ellenberg, Jan
中科院分区：
文献类型：
--
作者：
Lenart, Peter;Rabut, Gwenael;Daigle, Nathalie;Hand, Arthur R;Terasaki, Mark;Ellenberg, Jan
分解的核膜（NE）分析活海星卵母细胞使用的大小系列的荧光标记的葡聚糖，膜染料，和GFP标记的蛋白质的核孔复合物（NPC）和核纤层。细胞核的透化发生在两个连续的阶段。在第一阶段的NE变得越来越多的渗透分子直径高达40 nm，同时在10分钟的时间过程中的外围核孔组件的损失。NE保持完整的超微结构水平在这段时间。在阶段II中，NE在35秒内完全透化。这种快速的透化作用以波的形式从动物一侧的一个震中扩散到细胞核表面，并允许直径高达100 nm的颗粒自由扩散到细胞核中。虽然在光学显微镜水平下，板层和核膜似乎是完整的，但在第II阶段，通过电子显微镜可以清楚地看到NE的开窗。我们的结论是，NE的海星卵母细胞中的故障是由缓慢的顺序拆卸的NPC，然后由一个迅速蔓延的开窗的NE所造成的核孔从核膜仍然连接到板的去除。
Breakdown of the nuclear envelope (NE) was analyzed in live starfish oocytes using a size series of fluorescently labeled dextrans, membrane dyes, and GFP-tagged proteins of the nuclear pore complex (NPC) and the nuclear lamina. Permeabilization of the nucleus occurred in two sequential phases. In phase I the NE became increasingly permeable for molecules up to ∼40 nm in diameter, concurrent with a loss of peripheral nuclear pore components over a time course of 10 min. The NE remained intact on the ultrastructural level during this time. In phase II the NE was completely permeabilized within 35 s. This rapid permeabilization spread as a wave from one epicenter on the animal half across the nuclear surface and allowed free diffusion of particles up to ∼100 nm in diameter into the nucleus. While the lamina and nuclear membranes appeared intact at the light microscopic level, a fenestration of the NE was clearly visible by electron microscopy in phase II. We conclude that NE breakdown in starfish oocytes is triggered by slow sequential disassembly of the NPCs followed by a rapidly spreading fenestration of the NE caused by the removal of nuclear pores from nuclear membranes still attached to the lamina.