Mapping versatile QTL for soybean downy mildew resistance
Mapping versatile QTL for soybean downy mildew resistance
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DOI：
10.1007/s00122-018-3251-y
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发表时间：
2018-12
影响因子：
5.4
通讯作者：
F. Taguchi-Shiobara;K. Fujii;Takashi Sayama;Kaori Hirata;S. Kato;A. Kikuchi;Koji Takahashi;Masao Iwahashi;Chiaki Ikeda;K. Kosuge;Katsunori Okano;Masahiro Hayasaka;Y. Tsubokura;M. Ishimoto
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文献类型：
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作者：
F. Taguchi-Shiobara;K. Fujii;Takashi Sayama;Kaori Hirata;S. Kato;A. Kikuchi;Koji Takahashi;Masao Iwahashi;Chiaki Ikeda;K. Kosuge;Katsunori Okano;Masahiro Hayasaka;Y. Tsubokura;M. Ishimoto
KeymessageThree versatile QTL大豆霜霉病抗性在日本被检测到使用5个RIL群体，并确认使用重组固定对或回交line. AbstractDownny霜霉病降低大豆种子质量和大小。这在日本是一个问题，那里90%的大豆被用作食物。在美国，已报道了33个霜霉病小种，但在日本的小种分化尚不清楚。为了鉴定在关东和东北地区有效的霜霉病抗性的数量性状基因座（QTL），我们使用源自“Natto-shoryu”דTachinagaha”（NT）、“Natto-shoryu”דSuzumaru”、“Satonohohoemi”דFukuibuki”（SF）、“Kinusayaka”דCOL/秋田/2009/TARC/1、'和'YR-82' × 'Harosoy'在4年期间（2014-2017）。我们评估了自发发展的症状的RILs和应用112-233多态性标记，每个人口。在检测到的31个QTL中，有5个位于3个群体的第3染色体上，另外5个位于3个群体的第7染色体上。在一个群体中检测到了其他QTL，其中9个在不同年份检测到。在NT群体中，通过对关东和东北地区的9个重组固定对进行评估，在7号染色体上的3.0 Mb区域和18号染色体上的8.1 Mb区域检测到2个QTL。在SF群体中，在两个区域都检测到位于第8染色体上的QTL。通过回交将该QTL导入“Satonohohoemi”背景中，并在两个地区证实了其效应。总之，来自NT群体的第7和第18染色体上的两个QTL和来自SF群体的第8染色体上的一个QTL被证实在东北和关东地区都是有效的。
Key messageThree versatile QTL for soybean downy mildew resistance in Japan were detected using five RIL populations and confirmed using recombinant fixed pairs or a backcrossed line.AbstractDowny mildew reduces soybean seed quality and size. It is a problem in Japan, where 90% of soybean grown is used as food. In the USA, 33 downy mildew races have been reported, but race differentiation in Japan is unclear. To identify quantitative trait loci (QTL) for downy mildew resistance effective in the Kanto and Tohoku regions, we performed QTL analysis using five populations of recombinant inbred lines (RILs) originated from ‘Natto-shoryu’ × ‘Tachinagaha’ (NT), ‘Natto-shoryu’ × ‘Suzumaru’, ‘Satonohohoemi’ × ‘Fukuibuki’ (SF), ‘Kinusayaka’ × ‘COL/Akita/2009/TARC/1,’ and ‘YR-82’ × ‘Harosoy’ over a 4-year period (2014–2017). We evaluated spontaneously developed symptoms of the RILs and applied 112–233 polymorphic markers to each population. Out of 31 QTL detected, we found five on chromosome 3 in three populations and another five on chromosome 7 in three populations. Other QTL were detected in one population, nine of them in different years. In the NT population, two QTL were detected in a 3.0-Mb region on chromosome 7 and in an 8.1-Mb region on chromosome 18 by evaluating nine recombinant fixed pairs in both Kanto and Tohoku regions. In the SF population, a QTL on chromosome 8 was detected in both regions. This QTL was introduced into the ‘Satonohohoemi’ background by backcrossing, and its effect was confirmed in both regions. In summary, two QTL on chromosomes 7 and 18 from the NT population and one QTL on chromosome 8 from the SF population were confirmed to be effective in both Tohoku and Kanto regions.