The osmotic migration of cells in a solute gradient
The osmotic migration of cells in a solute gradient
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DOI：
10.1016/s0006-3495(99)76977-8
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发表时间：
1999-09-01
影响因子：
3.4
通讯作者：
Tryggvason, G
中科院分区：
文献类型：
--
作者：
Jaeger, M;Carin, M;Tryggvason, G
研究了非均匀溶质浓度对水通过简单模型细胞边界的渗透输送的影响。导出了在溶质快速扩散极限下单个胞体运动的两个常微分方程组，并得到了一种特殊情况的解析解。建立了一个二维有限元模型来模拟更一般的情况（有限扩散速率，由凝固锋引起的溶质梯度）。结果表明，由于水通过细胞边界的不均匀通量，细胞向溶质浓度较低的区域移动。这一机制显然以前没有被讨论过。在所有相关参数都已知的情况下，这种影响对红细胞的影响很小。然而，我们表明，它随着细胞大小和膜通透性的增加而增加，因此这种效应对较大的细胞可能很重要。所提出的有限元模型在研究细胞对渗透应力的反应以及细胞与凝固锋的相互作用方面也应具有其他应用。这些研究对于优化冷冻保存工艺具有重要意义。
The effect of a nonuniform solute concentration on the osmotic transport of water through the boundaries of a simple model cell is investigated. A system of two ordinary differential equations is derived for the motion of a single cell in the limit of a fast solute diffusion, and an analytic solution is obtained for one special case. A two-dimensional finite element model has been developed to simulate the more general case (finite diffusion rates, solute gradient induced by a solidification front). It is shown that the cell moves to regions of lower solute concentration due to the uneven flux of water through the cell boundaries. This mechanism has apparently not been discussed previously. The magnitude of this effect is small for red blood cells, the case in which all of the relevant parameters are known. We show, however, that it increases with cell size and membrane permeability, so this effect could be important for larger cells. The finite element model presented should also have other applications in the study of the response of cells to an osmotic stress and for the interaction of cells and solidification fronts. Such investigations are of major relevance for the optimization of cryopreservation processes.