Distribution profiles of membrane type-1 matrix metalloproteinase (MT1-MMP), matrix metalloproteinase-2 (MMP-2) and cyclooxygenase-2 (COX-2) in rabbit atherosclerosis: Comparison with plaque instability analysis
Distribution profiles of membrane type-1 matrix metalloproteinase (MT1-MMP), matrix metalloproteinase-2 (MMP-2) and cyclooxygenase-2 (COX-2) in rabbit atherosclerosis: Comparison with plaque instability analysis
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DOI：
10.1248/bpb.30.1634
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发表时间：
2007-09-01
影响因子：
2
通讯作者：
Saji, Hideo
中科院分区：
文献类型：
--
作者：
Kuge, Yuji;Takai, Nozomi;Saji, Hideo
背景资料：尽管越来越多的证据表明膜型基质金属蛋白酶（MT 1-MMP）、基质金属蛋白酶-2（MMP-2）和环氧合酶-2（考克斯-2）参与动脉粥样硬化的发病机制，但这些酶之间的可能联系仍不清楚。因此，我们研究了MT 1-MMP，MMP-2，和考克斯-2在高胆固醇血症（WHIFILM 1）兔动脉粥样硬化病变的免疫组织学分布。方法和结果：取4只WHHLMI兔的升弓段和胸主动脉60个横断面，用免疫组织化学方法检测MT 1-MMP、MMP-2和考克斯-2的分布。MT 1-MMP和MMP-2染色在动脉粥样硬化病变的巨噬细胞丰富区域显著观察到，并且与形态脆弱性呈正相关（MT 1-MMP r=0.63; MMP-2 r=0.60; p
Background: Despite increasing evidence that membrane type I matrix metalloproteinase (MT1-MMP), matrix metalloproteinase-2 (MMP-2), and cyclooxygenase-2 (COX-2) are involved in the pathogenesis of atherosclerosis, the possible links among these enzymes remain unclear. Accordingly, we investigated the distribution of MT1-MMP, MMP-2, and COX-2 immunohistologically in the atherosclerotic lesions of hypercholesterolemic (WHIFILM1) rabbits. Methods and Results: Distribution of MT1-MMP, MMP-2, and COX-2 was examined by immunohistochemical staining using sixty cross sections of the ascending-arch and thoracic aortas prepared from 4 WHHLMI rabbits. MT1-MMP and MMP-2 staining was prominently observed in the macrophage-rich regions of the atheromatous lesions, and was positively correlated with morphological vulnerability (r=0.63 for MT1-MMP; r=0.60 for MMP-2; p