THE CELLULAR-LOCALIZATION OF ADENOSINE RECEPTORS IN RAT NEOSTRIATUM
THE CELLULAR-LOCALIZATION OF ADENOSINE RECEPTORS IN RAT NEOSTRIATUM
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DOI：
10.1016/0306-4522(89)90011-0
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发表时间：
1989-01-01
期刊：
影响因子：
3.3
通讯作者：
REDDINGTON, M
中科院分区：
文献类型：
--
作者：
ALEXANDER, SP;REDDINGTON, M
使用定量放射自显影的配体结合位点结合特定的神经元通路的病变，A1和A2腺苷受体的细胞位置，以及第三个结合位点的腺苷受体配体，[3 H] N-乙基羧酰胺腺苷，和核苷转运蛋白进行了研究，在大鼠新纹状体。纹状体内红藻氨酸管理导致的损失50%的A1腺苷受体和几乎废除配体结合A2受体。一个小的减少[3 H]环己基腺苷结合纹状体A1受体后，发现病变的皮质纹状体输入。A2受体位点不受该处理的影响。破坏多巴胺能神经元使用6-羟基多巴胺或中缝纹状体的多巴胺能输入使用5，7-二羟色胺既不影响A1也不影响A2结合位点。这些结果表明，本地化的A1和A2腺苷受体的神经元内在的新纹状体和可能突触后多巴胺能输入。此外，[3 H] N-乙基甲酰氨基腺苷的结合位点，这是不受腺苷受体激动剂，R-苯基异丙基腺苷，也被部分废除后红藻氨酸注射。相比之下，没有显着的变化，核苷转运蛋白配体的结合，[3 H]nitrobenzylthioinosine，观察后的任何病变，表明该网站与各种细胞类型的广泛关联。
Using quantitative autoradiography of ligand binding sites combined with lesions of specific neuronal pathways, the cellular locations of A1 and A2 adenosine receptors, as well as a third binding site for the adenosine receptor ligand, [3H]N-ethylcarboxamidoadenosine, and a nucleoside transporter were investigated in rat neostriatum. Intrastriatal kainic acid administration resulted in the loss of 50% of A1 adenosine receptors and virtually abolished ligand binding to A2 receptors. A small reduction in [3H]cyclohexyladenosine binding to striatal A1 receptors was found after lesioning the corticostriatal input. A2 receptor sites were unaffected by this treatment. Destruction of dopaminergic neurons using 6-hydroxydopamine or the raphestriatal serotoninergic input using 5,7-dihydroxytryptamine affected neither A1 nor A2 binding sites. These results indicate the localization of both A1 and A2 adenosine receptors on neurons intrinsic to the neostriatum and probably postsynaptic to the dopaminergic input. In addition, a binding site for [3H]N-ethylcarboxamidoadenosine which is not affected by the adenosine receptor agonist, R-phenylisopropyladenosine, was also partly abolished after kainic acid injection. In contrast, no significant change in the binding of the nucleoside transporter ligand, [3H]nitrobenzylthioinosine, was observed after any lesions, indicating the widespread association of this site with various cell types.