Mechanosensitivity of mammalian auditory hair cells in vitro
Mechanosensitivity of mammalian auditory hair cells in vitro
复制标题
哺乳动物听觉毛细胞的体外机械敏感性
作者：
I. J. Russell;G. P. Richardson;A. Cody
从麻醉哺乳动物耳蜗体外记录的细胞内反应表明，机械感受性内毛细胞和外毛细胞被急剧调谐，解释了听觉神经中传入纤维的许多特性1 -4。然而，在体内不可能直接测量对这些细胞的兴奋性机械输入（它们的机械敏感性静纤毛的位移）5，6，从而确定受体电位与它们的静纤毛的位移之间的关系。作为一种手段，规避这一技术难题，我们已经开发了一种器官培养的小鼠耳蜗，在这里，我们描述的感受器电位产生的毛细胞在直接位移的静纤毛。
Intracellular responses recorded in vitro from the cochleas of anaesthetized mammals have shown that the mechanoreceptive inner and outer hair cells are sharply tuned, accounting for many of the properties of the afferent fibres in the auditory nerve1–4. However, in vivo it has not been possible to measure directly the excitatory mechanical input to these cells (the displacement of their mechanosensitive stereocilia)5,6 and thus to determine the relationship between the receptor potentials and displacement of their stereocilia. As a means of circumventing this technical difficulty, we have developed an organ culture of the mouse cochlea and here we describe the receptor potentials generated by the hair cells in response to direct displacement of their stereocilia.