Serum antibody does not account for differences in the severity of chronic respiratory disease caused by Mycoplasma pulmonis in LEW and F344 rats.
Serum antibody does not account for differences in the severity of chronic respiratory disease caused by Mycoplasma pulmonis in LEW and F344 rats.
复制标题
血清抗体不能解释 LEW 和 F344 大鼠中由肺支原体引起的慢性呼吸道疾病的严重程度差异。
DOI：
10.1128/iai.57.11.3570-3575.1989
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
Cassell,GH
中科院分区：
文献类型：
--
作者：
Simecka,JW;Davis,JK;Cassell,GH
肺支原体感染引起的大鼠慢性呼吸道疾病，有助于研究类似炎症性疾病的免疫机制，并为研究自然发生感染中全身免疫系统和粘膜免疫系统之间的相互作用提供了独特的机会。本研究检测了不同疾病进展和严重程度的大鼠菌株对肺支原体的血清抗体反应；LEW大鼠比F344大鼠出现更严重的疾病。血清抗体反应评估其水平，同型和抗原识别。与感染F344的大鼠相比，感染LEW的大鼠产生了更高或相同水平的主要类肺支原体血清抗体，这表明血清抗体反应的单独发展并不能消除病变，也不是LEW和F344大鼠疾病严重程度差异的原因。虽然LEW大鼠对免疫球蛋白G （IgG）的所有亚类均产生较高的应答，但LEW大鼠的特异性IgG应答主要由IgG1和IgG2a亚类组成，而IgG2b是F344大鼠IgG应答的主要成分。最后，LEW大鼠对肺支原体抗原的反应比F344大鼠更快，并且每种菌株最终识别的抗原没有差异，证实了先前的研究表明LEW大鼠对肺支原体的特定抗原没有反应性。
Chronic respiratory disease in rats, resulting from Mycoplasma pulmonis infection, is useful in the study of the immunological mechanisms in similar inflammatory diseases and provides a unique opportunity to study the interactions between systemic and mucosal immune systems in a naturally occurring infection. The present study examined the serum antibody responses to M. pulmonis in strains of rats which differ in disease progression and severity; LEW rats developed more severe disease than did F344 rats. Serum antibody responses were evaluated as to their levels, isotypes, and antigens recognized. Infected LEW rats produced greater or equal levels of the major classes of serum antibody to M. pulmonis than did infected F344 rats, suggesting that development of serum antibody responses alone does not resolve lesions and is not responsible for the difference in disease severity found in LEW and F344 rats. Although LEW rats produced higher responses in all subclasses of immunoglobulin G (IgG), the specific IgG response of LEW rats was composed predominately of IgG1 and IgG2a subclasses, while IgG2b was the major component of the IgG response in F344 rats. Finally, LEW rats responded more quickly to M. pulmonis antigens than did F344 rats, and there was no difference in the antigens eventually recognized by each strain, confirming previous work which suggested that LEW rats do not exhibit an unresponsiveness to a specific antigen(s) of M. pulmonis.