EVIDENCE THAT THE SPECTRIN NETWORK AND A NONOSMOTIC FORCE CONTROL THE FUSION PRODUCT MORPHOLOGY IN ELECTROFUSED ERYTHROCYTE-GHOSTS
EVIDENCE THAT THE SPECTRIN NETWORK AND A NONOSMOTIC FORCE CONTROL THE FUSION PRODUCT MORPHOLOGY IN ELECTROFUSED ERYTHROCYTE-GHOSTS
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DOI：
10.1016/s0006-3495(91)82140-3
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发表时间：
1991-11-01
影响因子：
3.4
通讯作者：
SOWERS, AE
中科院分区：
文献类型：
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作者：
CHERNOMORDIK, LV;SOWERS, AE
用光学显微镜和电子显微镜研究了通过介电泳法接触的红细胞幽灵共用的“接触区”中的膜面积通过电融合转化为融合产物。融合产品分为两类：(A)具有自由扩展的开放管腔的融合产品，其结束形式为“巨细胞形态”，并且具有大量的内部囊泡膜碎片；(B)具有长期稳定性但在幽灵-幽灵连接处具有平面横隔膜的线形多鬼链。超薄切片电子显微镜显示，这些平面隔膜均为双层隔膜，内有多个融合孔。已知的加热和低离子强度处理会使血影蛋白变性或分离，导致稳定的平面横隔膜溶解，从而迅速将多鬼链转变为巨细胞形态，从而表明如果血影蛋白完好无损，它会限制融合区直径的扩大。其他迹象表明，开放管腔的扩张似乎是由于一个或多个具有非渗透起源的膜特异力的结果，但如果接触区一侧的血影蛋白网络完好无损，这种扩张趋势可以被克服。
The conversion of the membrane area in the "contact zones" shared by erythrocyte ghosts held in contact by dielectrophoresis into a fusion product by electrofusion was studied by both light and electron microscopy. Fusion products fell into two categories: (a) those with a freely expanding open lumen which ended in the "giant cell morphology" and with considerable internal vesicle membrane fragments, and (b) linear chains of polyghosts with long term stability but having planar diaphragms at the ghost-ghost junctions. Thin section electron microscopy showed each of these planar diaphragms to be a double membrane septum multiply-perforated with fusion pores. Heat and low ionic strength treatments known to denature or detach spectrin caused the stable planar diaphragms to dissolve, thereby quickly converting the polyghost chains to the giant cell morphology, thereby suggesting that spectrin restricts fusion zone diameter expansion if it is intact. Other indications suggest that the expansion of the open lumens appears to take place as a result of one or more membrane-specific forces with a nonosmotic origin but this tendency to expansion can be overcome if the spectrin network on only one side of a contact zone is intact.