Characterization of the hemolytic activity of Riemerella anatipestifer
Characterization of the hemolytic activity of Riemerella anatipestifer
复制标题
鸭疫里默氏菌溶血活性的表征
DOI：
10.1099/mic.0.000896
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发表时间：
2020-05-01
期刊：
影响因子：
2.8
通讯作者：
Hu, Qinghai
中科院分区：
文献类型：
--
作者：
Gong, Yanshan;Yang, Yongsheng;Hu, Qinghai
鸭疫里默氏杆菌感染给世界养鸭业造成严重的经济损失。急性败血症和高血液细菌负荷在R。鸭疫病毒感染鸭表明，R.鸭瘟病菌可能能够通过溶解鸭红细胞来获得铁和其他营养物质，以支持其在血液中的快速生长和增殖。然而，到目前为止，对R.鸭瘟病菌对鸭红细胞的作用。在本研究中，52个R.鸭瘟病菌菌株在鸭血琼脂上显示溶血活性，而所有测试的DBA（+）（在鸭血琼脂上具有溶血活性）和DBA-菌株在鸭红细胞中产生孔，在孵育24小时后的液体溶血测定中具有4.35-9.03%的溶血活性。所有供试菌株的浓缩培养上清均用HPLC法测定。鸭疫病毒株和dba（+）R.鸭瘟病菌株在鸭血琼脂上表现出溶血活性。这些结果以及某些dba（+）和dba-R的半数致死量（LD 50）。鸭疫沙门氏菌的溶血力与雏鸭体内鸭疫沙门氏菌的溶血力无直接关系。鸭血琼脂上的鸭瘟病菌及其毒力。
Riemerella anatipestifer infection causes serious economic losses in the duck industry worldwide. Acute septicemia and high blood bacterial loading in R. anatipestifer infected ducks indicate that R. anatipestifer may be able to obtain iron and other nutrients by lysing duck erythrocytes to support its rapid growth and proliferation in the blood. However, so far, little is known about the hemolytic activity of R. anatipestifer to duck erythrocytes. In this study, 29 of 52 R. anatipestifer strains showed hemolytic activity on duck blood agar, whereas all the tested dba(+) (with hemolytic activity on duck blood agar) and dba- strains created pores in the duck red blood cells, with 4.35-9.03% hemolytic activity in a liquid hemolysis assay after incubation for 24 h. The concentrated culture supernatants of all the tested R. anatipestifer strains and the extracted outer membrane proteins (OMPs) from dba(+) R. anatipestifer strains showed hemolytic activity on duck blood agar. These results, together with the median lethal dose (LD50) of some dba(+) and dba- R. anatipestifer strains in ducklings, suggested that there was no direct relationship between the hemolytic capacity of R. anatipestifer on duck blood agar and its virulence.