Detection of homocysteine and cysteine
Detection of homocysteine and cysteine
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DOI：
10.1021/ja054962n
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发表时间：
2005-11-16
影响因子：
15
通讯作者：
Strongin, RM
中科院分区：
文献类型：
--
作者：
Wang, WH;Rusin, O;Strongin, RM
高水平的同型半胱氨酸（Hey，1）是心血管疾病、阿尔茨海默病、神经管缺陷和骨质疏松症的危险因素。1和半胱氨酸（Cys，3）都与神经毒性有关。1和3参与疾病状态的生化机制相对不清楚。在这里，我们描述了简单的方法，用于检测在可见光谱区的最高选择性，而不使用生化技术或制备分离。简单的方法和容易获得的试剂允许在生理相关水平的范围内检测Cys和Hey。报道了一种新的生物硫醇的高效液相色谱柱后检测方法。潜在的生物医学相关性的化学机制参与检测的1。
At elevated levels, homocysteine (Hey, 1) is a risk factor for cardiovascular diseases, Alzheimer's disease, neural tube defects, and osteoporosis. Both 1 and cysteine (Cys, 3) are linked to neurotoxicity. The biochemical mechanisms by which 1 and 3 are involved in disease states are relatively unclear. Herein, we describe simple methods for detecting either Hey or Cys in the visible spectral region with the highest selectivity reported to date without using biochemical techniques or preparative separations. Simple methods and readily available reagents allow for the detection of Cys and Hey in the range of their physiologically relevant levels. New HPLC postcolumn detection methods for biological thiols are reported. The potential biomedical relevance of the chemical mechanisms involved in the detection of 1 is described.