Cold shock response in mammalian cells.
Cold shock response in mammalian cells.
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DOI：
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发表时间：
1999-11
影响因子：
1.2
通讯作者：
J. Fujita
中科院分区：
文献类型：
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作者：
J. Fujita
与细菌和植物相比，冷休克反应在哺乳动物中很少引起注意，除了在一些领域，如适应性产热，耐寒性，细胞和器官的储存，以及最近，治疗脑损伤和蛋白质生产。在细胞水平上，哺乳动物细胞的一些反应与微生物相似;冷应激改变细胞膜的脂质组成，抑制蛋白质合成和细胞增殖的速率。虽然以前的研究大多涉及低于20摄氏度的温度，但轻度低温（32摄氏度）可以改变细胞对随后压力的反应，如HSP 110家族成员APG-1所示。此外，32摄氏度诱导CIRP（冷诱导RNA结合蛋白）的表达，这是哺乳动物细胞中鉴定的第一种冷休克蛋白，在37摄氏度时没有恢复。与HSP类似，CIRP也在37 ℃表达，并在发育过程中受到调节，可能作为RNA伴侣发挥作用。哺乳动物细胞在32摄氏度时代谢活跃，细胞可以存活并以与37摄氏度不同的策略应对压力。哺乳动物细胞在32摄氏度的细胞和分子生物学是一个新的领域，预计将有相当大的影响医学科学和可能的生物技术。
Compared to bacteria and plants, the cold shock response has attracted little attention in mammals except in some areas such as adaptive thermogenesis, cold tolerance, storage of cells and organs, and recently, treatment of brain damage and protein production. At the cellular level, some responses of mammalian cells are similar to microorganisms; cold stress changes the lipid composition of cellular membranes, and suppresses the rate of protein synthesis and cell proliferation. Although previous studies have mostly dealt with temperatures below 20 degrees C, mild hypothermia (32 degrees C) can change the cell's response to subsequent stresses as exemplified by APG-1, a member of the HSP110 family. Furthermore, 32 degrees C induces expression of CIRP (cold-inducible RNA-binding protein), the first cold shock protein identified in mammalian cells, without recovery at 37 degrees C. Remniscent of HSP, CIRP is also expressed at 37 degrees C and developmentary regulated, possibly working as an RNA chaperone. Mammalian cells are metabolically active at 32 degrees C, and cells may survive and respond to stresses with different strategies from those at 37 degrees C. Cellular and molecular biology of mammalian cells at 32 degrees C is a new area expected to have considerable implications for medical sciences and possibly biotechnology.