Leukocyte and Platelet Rich Plasma (L-PRP) Versus Leukocyte and Platelet Rich Fibrin (L-PRF) For Articular Cartilage Repair of the Knee: A Comparative Evaluation in an Animal Model
Leukocyte and Platelet Rich Plasma (L-PRP) Versus Leukocyte and Platelet Rich Fibrin (L-PRF) For Articular Cartilage Repair of the Knee: A Comparative Evaluation in an Animal Model
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DOI：
10.5812/ircmj.19594
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发表时间：
2015-10-01
影响因子：
0.4
通讯作者：
Fakhrjou, Ashraf
中科院分区：
文献类型：
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作者：
Kazemi, Davoud;Fakhrjou, Ashraf
背景：膝关节软骨损伤是导致骨性关节炎最严重的损伤之一，其原因是软骨组织再生能力有限。应用含有促进软骨愈合所需生长因子的血小板浓缩物是近年来出现的一种新的治疗方法。目的：比较两种不同类型的血小板浓缩物在急性膝关节软骨损伤动物模型中的治疗效果。材料和方法：选用18只成年伊朗混血雄性犬进行实验研究。在犬双后肢股骨髁负重区建立全层关节软骨缺损模型(直径6 mm，深5 mm)，随机选择12只犬进行治疗，分别植入L-PRP和L-PRF修复右、左后肢软骨缺损，另6只犬为对照组。分别于术后4、16、24周对动物实施安乐死，并对修复组织进行大体和显微观察。结果：在每个采样时间，处理组和对照组的平均宏观评分均高于对照组，分别在4周和16周时，L修复组和对照组的宏观评分分别为10.13和8.37，L修复组和对照组的宏观评分分别为10和8.5，差异有统计学意义(P<；0.05)。在第4周(9.87vs.7.62)和第16周(13.38vs.11)，L-PRP处理组和对照组的平均总显微镜评分也有类似的趋势，差异有统计学意义(P<；0.05)。结论：L-PRP和L-PRF均能有效促进膝关节软骨缺损的愈合。
Background: Articular cartilage injuries of the knee are among the most debilitating injuries leading to osteoarthritis due to limited regenerative capability of cartilaginous tissue. The use of platelet concentrates containing necessary growth factors for cartilage healing has recently emerged as a new treatment method.Objectives: The efficacy of two types of different platelet concentrates were compared in the treatment of acute articular cartilage injuries of the knee in an animal model.Materials and Methods: Eighteen adult Iranian mixed breed male dogs were used to conduct this experimental study. Full thickness articular cartilage defects (diameter 6 mm, depth 5 mm)were created in the weight bearing area of femoral condyles of both hind limbs in all dogs (n = 72).Twelve dogs were randomly selected to receive treatment and their right and left hind limb defects were treated by L-PRP and L-PRF implantation respectively, while no treatment was undertaken in six other dogs as controls. The animals were euthanized at 4, 16 and 24 weeks following surgery and the resultant repair tissue was investigated macroscopically and microscopically. At each sampling time, 4 treated dogs and 2 control dogs were euthanized, therefore 8 defects per group were evaluated.Results: Mean macroscopic scores of the treated defects were higher than the controls at all sampling times with significant differences (P < 0.05) observed between L-PRF treated and control defects (10.13 vs. 8.37) and L-PRP treated and control defects (10 vs. 8.5) at 4 and 16 weeks, respectively. A similar trend in mean total microscopic scores was observed with a significant difference (P < 0.05) between L-PRP treated and control defects at 4 (9.87 vs. 7.62) and 16 (13.38 vs. 11) weeks. No significant difference was observed between the platelet concentrate treated defects in either mean macroscopic scores or mean total microscopic scores.Conclusions: Both L-PRP and L-PRF could be used to effectively promote the healing of articular cartilage defects of the knee.