Bioactive glass and high-intensity lasers as a promising treatment for dentin hypersensitivity: An in vitro study
Bioactive glass and high-intensity lasers as a promising treatment for dentin hypersensitivity: An in vitro study
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DOI：
10.1002/jbm.b.34446
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发表时间：
2019-08-05
影响因子：
3.4
通讯作者：
Marques, Marcia M.
中科院分区：
文献类型：
--
作者：
Lee, Ester M. R.;Borges, Roger;Marques, Marcia M.
本体外研究旨在分析生物活性玻璃（BG）糊剂（BG/Ac）应用于高功率激光照射或未照射后过敏的人牙本质样表面的物理和化学特性。牙本质标本用17%乙二胺四乙酸（EDTA）溶液处理，以模拟过敏牙本质，然后提交给钕：钇铝石榴石（Nd：YAG）激光或CO2激光照射之前和之后的应用BG/Ac。采用X射线衍射、傅里叶变换红外光谱、扫描电子显微镜和能量色散X射线光谱进行表征。结果提示，BG/Ac单独使用对牙本质小管有一定的阻塞作用。Nd：YAG激光照射减少牙本质小管的开放，而胶原结构没有变化。CO2激光照射引起牙本质沿着熔化和再固化，并伴有裂纹和胶原纤维的化学变化。然而，当BG/Ac糊剂用激光照射时，玻璃与牙本质界面之间的一系列表面反应导致形成无定形羟基磷灰石层，类似于正常牙本质的无机组分。此外，BG/Ac能够防止由CO2照射引起的胶原纤维的裂纹和降解的形成。总体而言，本研究支持应用高功率激光照射的BG/AC糊剂可以代表一种有效且持久的治疗牙本质过敏症的方法。
This in vitro study aimed to analyze the physical and chemical characteristics of the hypersensitive human dentin-like surface after application of a bioactive glass (BG) paste (BG/Ac) irradiated or not with high-power lasers. Dentin specimens were treated with 17% Ethylenediamine tetraacetic acid (EDTA) solution to mimic a hypersensitive dentin and then submitted to neodymium: yttrium-aluminum-garnet (Nd:YAG) laser or CO2 laser irradiation prior and after application of BG/Ac. Characterizations were performed by using X-ray diffraction, Fourier transformed infrared spectroscopy, scanning electron microscopy, and energy dispersive X-ray spectroscopy. The results suggested that application of BG/Ac by itself caused some obstructions of dentinal tubules. Nd:YAG laser irradiation reduced the opening of the dentinal tubules with no changes in the collagen structure. CO2 laser irradiation caused dentin melting and resolidification along with cracks and chemical changes in collagen fibers. However, when BG/Ac paste was irradiated with lasers, a sequence of surface reactions between glass and dentin interface led to the formation of an amorphous hydroxyapatite layer, similar to that of an inorganic component of the normal dentin. Moreover, BG/Ac was able to prevent the formation of cracks and degradation of collagen fibers caused by CO2 irradiation. Overall, this study supports that application of BG/Ac paste irradiated by high-power laser could represent an effective and long-lasting therapeutic approach for dentin hypersensitivity.