Classification of a new phytoplasmas subgroup 16SrII-W associated with Crotalaria witches' broom diseases in Oman based on multigene sequence analysis.
Classification of a new phytoplasmas subgroup 16SrII-W associated with Crotalaria witches' broom diseases in Oman based on multigene sequence analysis.
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DOI：
10.1186/s12866-017-1130-3
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发表时间：
2017-11-25
期刊：
影响因子：
4.2
通讯作者：
Al-Sadi AM
中科院分区：
文献类型：
--
作者：
Al-Subhi A;Hogenhout SA;Al-Yahyai RA;Al-Sadi AM
埃及猪屎豆（Crotalaria aegyptiaca）是一种低矮灌木，常见于干河沙漠的沙质土壤中，在阿曼所有地区都有发现。进行了植原体病害调查。 2015年在阿曼北部地区的野生地区进行调查时，在埃及念珠菌植物上观察到植原体感染的典型症状。受感染的植物的芽和小叶子过度增殖。通过使用透射电子显微镜 (TEM) 证实了有症状的埃及念珠菌叶子样品的韧皮部组织中存在植原体。此外，还从有症状的 C. aegyptiaca 叶子样本和 Orosius sp. 中提取了 DNA。使用针对 16S rDNA、secA、tuf 和 imp 以及 SAP11 基因的植原体特异性引物，通过 PCR 测试叶蝉。所有样本的 PCR 扩增均产生了预期产物，但无症状植物样本则没有。 4个基因(16S rDNA、secA、tuf和imp)的序列相似性和系统发育树分析表明，来自阿曼的猪屎豆植原体与花生WB(16SrII)的进化枝接近蚕豆叶状体(16SrII-C)、棉花叶状体和植原体(16SrII-F)和Candidatus橙叶植原体（16SrII-B）。然而，猪屎豆的植原体与属于花生 WB 组的所有其他植原体处于不同的亚支系中。将16SrII-A、-B、-D亚群菌原体SAP11基因的特异性引物组合与猪屎霉植原体进行测试，未扩增出PCR产物，这表明猪屎霉植原体的SAP11与其他植原体的SAP11不同。我们建议根据 16S rRNA、secA、imp、tuf 和 SAP11 基因的序列分析，将来自阿曼的猪屎豆归入一个新的谱系 16SrII-W 亚组。据我们所知，这是全球范围内首次报道 16SrII 类植原体感染埃及念珠菌。本文的在线版本 (10.1186/s12866-017-1130-3) 包含补充材料，可供授权用户使用。
Crotalaria aegyptiaca, a low shrub is commonly observed in the sandy soils of wadis desert and is found throughout all regions in Oman. A survey for phytoplasma diseases was conducted. During a survey in a wild area in the northern regions of Oman in 2015, typical symptoms of phytoplasma infection were observed on C. aegyptiaca plants. The infected plants showed an excessive proliferation of their shoots and small leaves. The presence of phytoplasma in the phloem tissue of symptomatic C. aegyptiaca leaf samples was confirmed by using Transmission Electron Microscopy (TEM). In addition the extracted DNA from symptomatic C. aegyptiaca leaf samples and Orosius sp. leafhoppers were tested by PCR using phytoplasma specific primers for the 16S rDNA, secA, tuf and imp, and SAP11 genes. The PCR amplifications from all samples yielded the expected products, but not from asymptomatic plant samples. Sequence similarity and phylogenetic tree analyses of four genes (16S rDNA, secA, tuf and imp) showed that Crotalaria witches’ broom phytoplasmas from Oman is placed with the clade of Peanut WB (16SrII) close to Fava bean phyllody (16SrII-C), Cotton phyllody and phytoplasmas (16SrII-F), and Candidatus Phytoplasma aurantifolia’ (16SrII-B). However, the Crotalaria’s phytoplasma was in a separate sub-clade from all the other phytoplasmas belonging to Peanut WB group. The combination of specific primers for the SAP11 gene of 16SrII-A, −B, and -D subgroup pytoplasmas were tested against Crotalaria witches’ broom phytoplasmas and no PCR product was amplified, which suggests that the SAP11 of Crotalaria phytoplasma is different from the SAP11 of the other phytoplasmas. We propose to assign the Crotalaria witches’ broom from Oman in a new lineage 16SrII-W subgroup depending on the sequences analysis of 16S rRNA, secA, imp, tuf, and SAP11 genes. To our knowledge, this is the first report of phytoplasmas of the 16SrII group infecting C. aegyptiaca worldwide. The online version of this article (10.1186/s12866-017-1130-3) contains supplementary material, which is available to authorized users.
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影响因子：
2.2
作者：
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通讯作者：
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