BABA application improves soybean resistance to aphid through activation of phenylpropanoid metabolism and callose deposition
BABA application improves soybean resistance to aphid through activation of phenylpropanoid metabolism and callose deposition
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BABA 应用通过激活苯丙素代谢和胼胝质沉积提高大豆对蚜虫的抗性
DOI：
10.1002/ps.5526
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发表时间：
2019
影响因子：
4.1
通讯作者：
Tianlong Wu
中科院分区：
文献类型：
--
作者：
Luming Yao;Yunpeng Zhong;Biao Wang;Junhui Yan;Tianlong Wu
背景-氨基丁酸（BABA）赋予植物对广泛的生物和非生物胁迫的抗性。大豆蚜虫原产于东亚，是大豆的主要害虫。异黄酮途径和木质素途径都是一般苯丙素途径的重要分支，可能与大豆蚜虫的抗性有关。然而，关于苯丙素途径在BABA应用诱导的SBA防御反应中的作用知之甚少。结果BABA有效增强了大豆对大豆蚜虫甘氨酸蚜虫的抗性。与显著提高异黄酮含量，尤其是染料木素含量一致的是，BABA上调了相关的生物合成基因。木质素是另一种重要的节肢动物防御成分，木质素的积累水平较高，4个木质素生物合成相关基因也被激活。此外，施用BABA增加了SBA侵染的幼苗中胼胝质合成酶基因的表达，并增加了胼胝质沉积。在非笼养和笼养试验中，BABA处理的幼苗中SBA数量显著降低。结论施用BABA对大豆抗蚜有明显的正向作用，其防御作用与大豆异黄酮合成和胼胝质沉积的增强有关。©2019化学工业协会
BACKGROUNDBeta‐aminobutyric acid (BABA) confer plant resistance to a broad spectrum of biotic and abiotic stresses. The soybean aphid (SBA), is native to eastern Asia and is a predominant insect pest of soybean. Both isoflavone and lignin pathway are important branches of the general phenylpropanoid pathway, which would be likely associated with resistance against soybean aphid. However, little is known about the role of the phenylpropanoid pathway in defense response to SBA as induced by BABA application.RESULTSThe application of BABA effectively enhanced soybean resistance againstAphis glycines, the soybean aphid. Consistent with significantly increased content of isoflavones, especially genistein, the related biosynthetic genes were upregulated by use of BABA. Lignin, another important defense component against arthropods, accumulated at a high level and four lignin biosynthesis related genes were also activated. Additionally, BABA application augmented the expression of callose synthase genes and increased callose deposition in SBA‐infested seedlings. In non‐caged and caged tests, SBA numbers were significantly reduced in BABA‐treated seedlings.CONCLUSIONThese results demonstrate that application of BABA has an obvious positive effect on soybean resistance to aphids, and this defense response partly depends on the potentiation of isoflavone biosynthesis and callose deposition. © 2019 Society of Chemical Industry