DIMINISHED PROTECTION AGAINST COPPER-INDUCED LIPID-PEROXIDATION BY CORD-BLOOD PLASMA OF PRETERM AND TERM INFANTS
DIMINISHED PROTECTION AGAINST COPPER-INDUCED LIPID-PEROXIDATION BY CORD-BLOOD PLASMA OF PRETERM AND TERM INFANTS
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DOI：
10.1177/0148607195019005373
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发表时间：
1995-09-01
影响因子：
3.4
通讯作者：
BERGER, HM
中科院分区：
文献类型：
--
作者：
LINDEMAN, JHN;LENTJES, EGWM;BERGER, HM
背景：新生儿血浆对铁诱导的脂质过氧化反应的抑制能力低于老年患者血浆。铜，也是一种强氧化剂，可能会积累在生病的婴儿，因为过量摄入或减少排泄。我们在体外评估了新生儿血浆抑制铜诱导的过氧化损伤的能力。方法：用硫代巴比妥酸法测定CuCl 2诱导的磷脂脂质体的过氧化作用。比较了成人血浆角静脉血和婴儿脐带血的抗过氧化能力。还测量了铜结合血浆蛋白、白蛋白和血浆铜蓝蛋白的水平。结果：保护铜诱导的脂质过氧化作用，成人血浆相比，新生儿血浆高得多。尽管早产儿的白蛋白和血浆铜蓝蛋白水平较低，但早产儿血浆的保护作用高于足月儿。结论：出生时，婴儿在体外抑制铜诱导的氧化损伤的能力有限。产后研究需要评估成熟和营养对这些发现的影响及其在活性氧引起的疾病中的相关性。
Background: Blood plasma of neonates is less able to inhibit iron induced lipid peroxidation than plasma of older patients. Copper, also a powerful pro-oxidant, may accumulate in ill babies because of excess intake or decreased excretion. We assessed in vitro the ability of plasma of neonates to inhibit copper induced peroxidation damage. Methods: Peroxidation of phospholipid liposomes, induced by CuCl2, was measured with a thiobarbituric acid assay. The ability of plasma horn venous blood of adults and cord blood of babies to inhibit peroxidation was compared. The levels of the copper binding plasma proteins, albumin and ceruloplasmin, were also measured. Results: Protection against copper induced lipid peroxidation was much higher with adult plasma compared to neonatal plasma. Despite their lower albumin and ceruloplasma levels the protection by the plasma of preterm babies was higher than that of the term babies. Conclusions: At birth, babies have a limited ability to inhibit copper induced oxidative damage in vitro. Postnatal studies are needing to assess the influence of maturation and nutrition on these findings and their relevance in diseases induced by reactive oxygen species.