The Mouse Intestinal Bacterial Collection (miBC) provides host-specific insight into cultured diversity and functional potential of the gut microbiota
The Mouse Intestinal Bacterial Collection (miBC) provides host-specific insight into cultured diversity and functional potential of the gut microbiota
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DOI：
10.1038/nmicrobiol.2016.131
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发表时间：
2016-10-01
影响因子：
28.3
通讯作者：
Clavel, Thomas
中科院分区：
文献类型：
--
作者：
Lagkouvardos, Ilias;Pukall, Ruediger;Clavel, Thomas
肠道细菌影响哺乳动物的生理，但许多类型的细菌仍然是未知的。此外，尽管小鼠模型广泛用于医学研究，但小鼠肠道细菌的参考菌株并不容易获得。这些是研究肠道微生物组及其与饮食和宿主相互作用的主要限制。因此，重要的是要详细研究肠道微生物群成员的多样性和功能，包括那些殖民小鼠肠道。为了解决这些问题，我们的目标是建立小鼠肠道细菌保藏中心（miBC），一个来自小鼠肠道的细菌菌株和相关基因组的公共储存库，并研究了定植的宿主特异性和基于序列的资源相关性。该系列包括代表新物种，属，甚至一个家庭的几个菌株。基因组分析表明，某些物种对小鼠肠道具有特异性，并且18种菌株的最小聚生体覆盖了宏基因组已知功能潜力的50-75%。目前的工作将支持未来对健康和疾病中微生物与宿主相互作用的研究，因为它将促进有针对性的定植和分子研究。该资源可在www.dsmz.de/miBC上获得。
Intestinal bacteria influence mammalian physiology, but many types of bacteria are still uncharacterized. Moreover, reference strains of mouse gut bacteria are not easily available, although mouse models are extensively used in medical research. These are major limitations for the investigation of intestinal microbiomes and their interactions with diet and host. It is thus important to study in detail the diversity and functions of gut microbiota members, including those colonizing the mouse intestine. To address these issues, we aimed at establishing the Mouse Intestinal Bacterial Collection (miBC), a public repository of bacterial strains and associated genomes from the mouse gut, and studied host-specificity of colonization and sequence-based relevance of the resource. The collection includes several strains representing novel species, genera and even one family. Genomic analyses showed that certain species are specific to the mouse intestine and that a minimal consortium of 18 strains covered 50-75% of the known functional potential of metagenomes. The present work will sustain future research on microbiota-host interactions in health and disease, as it will facilitate targeted colonization and molecular studies. The resource is available at www.dsmz.de/miBC.