Identification of differentially expressed genes in mouse spermatogenesis.
Identification of differentially expressed genes in mouse spermatogenesis.
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小鼠精子发生中差异表达基因的鉴定。
DOI：
10.1002/j.1939-4640.2003.tb03142.x
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发表时间：
2003
影响因子：
--
通讯作者：
Chan,Wai-Yee
中科院分区：
文献类型：
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作者：
Pang,AlanLY;Taylor,HClaire;Johnson,Warren;Alexander,Stefanie;Chen,Yali;Su,YanA;Li,Xiaoquan;Ravindranath,Neelakanta;Dym,Martin;Rennert,OwenM;Chan,Wai-Yee
互补DNA微阵列和定量聚合酶链反应被用来作为工具，发现差异表达的基因在小鼠在正常生理条件下，在不同阶段的男性生殖细胞的发展，即A型精原细胞，粗线期精母细胞，和圆形精子细胞。通过使用这种策略，我们确定了一组在精子发生中表现出差异表达模式的基因，这表明编码产物的特定功能发生在发育过程中。其中有几个基因以前不知道在睾丸中是活跃的，这表明这些基因在精子发生过程中的功能尚未被发现。许多被鉴定的基因以前没有被鉴定过。这项研究突出了新的目标，操纵解开精子发生的分子机制。
Complementary DNA microarray and quantitative polymerase chain reaction were used as tools for discovering genes that are differentially expressed in the mouse under normal physiological conditions at distinctive stages of male germ cell development, that is, type A spermatogonia, pachytene spermatocytes, and round spermatids. By using this strategy, we identified a set of genes exhibiting differential expression patterns in spermatogenesis, suggesting that specific functions of the encoded products occurred during the developmental process. Among them were several genes previously not known to be active in testis, which signified undiscovered functional roles of these genes during spermatogenesis. Many of the genes identified were not previously characterized. This study highlights new targets for manipulation to unravel the molecular mechanism of spermatogenesis.