Tissue Sampling Guides for Porcine Biomedical Models
Tissue Sampling Guides for Porcine Biomedical Models
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DOI：
10.1177/0192623316631023
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发表时间：
2016-04-01
影响因子：
1.5
通讯作者：
Blutke, Andreas
中科院分区：
文献类型：
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作者：
Albl, Barbara;Haesner, Serena;Blutke, Andreas
本文提供了转化医学研究中适用于猪动物模型的器官和组织取样指南。补充材料中提供了确定采样位置和数量的详细方案以及关于约50种不同猪器官和组织样本的方向、大小和修剪方向的建议。拟定的采样方案包括生成适合后续定性和定量分析的样本，包括冷冻组织学、石蜡和塑料组织学;免疫组织化学;原位杂交;电子显微镜;定量体视学以及DNA、RNA、蛋白质、代谢物和电解质的分子分析。关于计划的取样工作、时间和人员费用的程度，并根据预定的分析，提供不同的协议。对这些方案进行了调整，以适应（I）常规筛查，如一般毒性研究或基因表达模式或组织病理学器官改变分析中所用，（II）单个器官/组织的高级分析，以及（III）生物库项目中应用的大规模采样程序。为猪模型的研究提供了一个可靠的参考，所描述的方案将确保采样的效率，代表整个器官或组织的高质量样品的系统回收，以及研究内/研究间的可比性和结果的再现性。
This article provides guidelines for organ and tissue sampling adapted to porcine animal models in translational medical research. Detailed protocols for the determination of sampling locations and numbers as well as recommendations on the orientation, size, and trimming direction of samples from approximate to 50 different porcine organs and tissues are provided in the Supplementary Material. The proposed sampling protocols include the generation of samples suitable for subsequent qualitative and quantitative analyses, including cryohistology, paraffin, and plastic histology; immunohistochemistry; in situ hybridization; electron microscopy; and quantitative stereology as well as molecular analyses of DNA, RNA, proteins, metabolites, and electrolytes. With regard to the planned extent of sampling efforts, time, and personnel expenses, and dependent upon the scheduled analyses, different protocols are provided. These protocols are adjusted for (I) routine screenings, as used in general toxicity studies or in analyses of gene expression patterns or histopathological organ alterations, (II) advanced analyses of single organs/tissues, and (III) large-scale sampling procedures to be applied in biobank projects. Providing a robust reference for studies of porcine models, the described protocols will ensure the efficiency of sampling, the systematic recovery of high-quality samples representing the entire organ or tissue as well as the intra-/interstudy comparability and reproducibility of results.