Genetic engineering of resistance to bleaching herbicides affecting phytoene desaturase and lycopene cyclase in cyanobacterial carotenogenesis
Genetic engineering of resistance to bleaching herbicides affecting phytoene desaturase and lycopene cyclase in cyanobacterial carotenogenesis
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DOI：
10.1006/pest.1997.2261
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发表时间：
1997-01-01
影响因子：
4.7
通讯作者：
Boger, P
中科院分区：
文献类型：
--
作者：
Windhovel, U;Sandmann, G;Boger, P
类胡萝卜素生物合成途径的酶是漂白除草剂影响光合生物的色素组成的靶位点。蓝细菌代表了对影响高等植物胡萝卜素生成的除草剂抗性的基因工程的便利模型。因此，将非光合细菌噬夏欧文氏菌的基因crtl和crtY同时插入到蓝细菌聚球藻PCC 7942的基因组中。两种基因的产物，即八氢番茄红素去饱和酶和番茄红素环化酶，分别催化类胡萝卜素生物合成中的反应序列，从八氢番茄红素经由六氢番茄红素、ζ-胡萝卜素、脉孢烯、番茄红素和γ-胡萝卜素生成β-胡萝卜素。虽然植物型八氢番茄红素去饱和酶是漂白除草剂氟草酮的靶位点，但细菌酶对该化合物具有高度抗性。另一方面，植物和细菌来源的番茄红素环化酶。已显示被取代的三烷基胺抑制。我们证明，由此产生的减少环类胡萝卜素是伴随着漂白的叶绿素在光。测定由氟草酮和三烷基胺化合物BTS 6772 [2-（4-氯苯硫基）甲基二乙胺盐酸盐]引起的类胡萝卜素生物合成的体内抑制的摩尔I-50值，揭示了在蓝藻中对这两种除草剂的同时抗性。(C)北京：科学出版社.
Enzymes of the carotenoid biosynthetic pathway are target sites for bleaching herbicides affecting the pigment composition of photosynthetic organisms. Cyanobacteria represent convenient models for the genetic engineering of resistance to herbicides affecting carotenogenesis of higher plants. Therefore, the genes crtl and crtY of the nonphotosynthetic bacterium Erwinia uredovora were simultaneously inserted into the genome of the cyanobacterium Synechococcus PCC7942. The products of both genes, the enzymes phytoene desaturase and lycopene cyclase, respectively, catalyze the reaction sequence in carotenoid biosynthesis leading from phytoene to beta-carotene via phytofluene, zeta-carotene, neurosporene, lycopene, and gamma-carotene. While plant-type phytoene desaturases are target sites of the bleaching herbicide norflurazon, the bacterial enzyme is highly resistant to this compound. Lycopene cyclases of plant and bacterial origin, on the other hand. have been shown to be inhibited by substituted trialkylamines. We demonstrate that the resulting decrease of cyclic carotenoids was accompanied by bleaching of chlorophyll in the light. The determination of molar I-50 values for in vivo inhibition of carotenoid biosynthesis caused by norflurazon and by the trialkylamine compound BTS 6772 [2-(4-chlorophenylthio)methyldiethylamine hydrochloride] revealed a simultaneous resistance to these two herbicides in the cyanobacterial transformant strain. (C) 1997 Academic Press.