Endothelial cells: a source and target of oxidant damage.
Endothelial cells: a source and target of oxidant damage.
复制标题
内皮细胞：氧化损伤的来源和目标。
DOI：
10.1007/978-1-4684-5568-7_157
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Vann,JM
中科院分区：
文献类型：
--
作者：
Ryan,US;Vann,JM
由于内皮细胞位于血液和组织之间，因此非常适合接收血液和组织来源的信号。同样，内皮细胞及其产品处于战略位置，可以直接与血液细胞和分子以及潜在组织相互作用。我们现在知道，内皮细胞是高度代谢活跃和反应灵敏的细胞，它控制物质通过血管壁，并调节循环中生物活性分子的质量和数量。1内皮细胞与中性粒细胞和巨噬细胞等细胞类型的许多相互作用可能涉及内皮细胞作为氧化损伤的目标“(另见PA Ward，本卷)。然而，内皮细胞本身可以对刺激做出反应，向周围环境释放活性产物，包括氧代谢物。其中一些内皮衍生产品可能具有调节功能，如内皮衍生松弛因子(EDRF)及其对血小板和平滑肌细胞的作用，而另一些则可能被设计为一种细胞防御机制，例如对抗微生物感染。越来越多的证据表明，内皮细胞可以对各种各样的刺激做出同样广泛的反应。然而，不太清楚的是，内皮细胞如何将信号转化为反应。我们最近开始研究内皮细胞的信号转导机制。在这份报告中，我们研究了两个系统：1)激动剂诱导内皮细胞释放EDRF；2)产生超氧阴离子(02；)以响应细菌的摄入。
By virtue of their position interposed between the blood and the tissues, endothelial cells are ideally placed to receive both blood-borne and tissue-derived signals. By the same token, endothelial cells and their products are strategically situated to interact directly with cells and molecules of blood and with the underlying tissues. We now know that endothelial cells are highly metabolically active and responsive cells that control the passage of substances across the vascular wall and regulate the quality and quantity of circulating bioactive molecules. 1 Many of the interactions of endothelial cells with cell types such a neutrophils and macropha1e~ may involve the endothelial cell as the target of oxidant injury'(see also PA Ward, this volume). However, endothelial cells themselves can respond to stimuli to release active products, including oxygen metabolites, into the surroundings. Some of these endothelial-derived products may serve a regulatory function as in the case of endothelium-derived relaxing factor (EDRF), and its actions on platelets and smooth muscle cells, while others are presumably designed as a cellular defense mechanism against, for example, microbial infection. Evidence is accumulating to indicate that endothelial cells can respond to a wide variety of stimuli with an equally wide range of responses. However, what is less clear is how the endothelium translates the signal into the response. We have recently set out to determine the mechanisms of signal transduction in endothelial cells. In this report we examine two systems: 1) agonist-induced release of EDRF from endothelial cells and 2) production of superoxide anion (02;) in response to ingestion of bacteria.