Characteristic expression of MSX1, MSX2, TBX2 and ENTPD1 in dental pulp cells
Characteristic expression of MSX1, MSX2, TBX2 and ENTPD1 in dental pulp cells
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DOI：
10.3892/br.2015.456
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发表时间：
2015-07-01
影响因子：
2.3
通讯作者：
Kato, Yukio
中科院分区：
文献类型：
--
作者：
Fujii, Sakiko;Fujimoto, Katsumi;Kato, Yukio
牙髓细胞在再生医学中是一种很有前途的可移植细胞来源。然而，DPC在分子水平上还没有得到充分的表征。本研究的目的是通过几个DPC特征基因的表达来区分不同来源的间充质干细胞(MSCs)、成纤维细胞(FBS)和其他细胞。采用组织块法或酶消化法从人牙髓组织中分离出DPC，用含10%血清或7.5%富血小板血浆的培养液培养。从细胞和牙髓组织标本中提取RNA。用DNA芯片和定量聚合酶链式反应检测基因表达水平。DPC的MSH同源盒1、MSH同源盒2、T盒2和胞外核苷三磷酸二磷酸水解酶1mRNA水平高于下列细胞类型的水平：骨髓、滑膜和脂肪组织来源的MSCs，以及FBS、成骨细胞、脂肪细胞和软骨细胞。无论供者的年龄、牙齿类型和培养介质如何，DPC中的表达增强都是一致的。此外，这些基因在活体牙髓组织中高水平表达。综上所述，该基因集可用于基础研究和牙髓细胞再生治疗中的DPC的鉴定和表征。
Dental pulp cells (DPCs) are a promising source of transplantable cells in regenerative medicine. However, DPCs have not been fully characterized at the molecular level. The aim of the present study was to distinguish DPCs from various source-derived mesenchymal stem cells (MSCs), fibroblasts (FBs) and other cells by the expression of several DPC-characteristic genes. DPCs were isolated from human pulp tissues by the explant method or the enzyme digestion method, and maintained with media containing 10% serum or 7.5% platelet-rich plasma. RNA was isolated from the cells and from dental pulp tissue specimens. The mRNA levels were determined by DNA microarray and quantitative polymerase chain reaction analyses. The msh homeobox 1, msh homeobox 2, T-box 2 and ectonucleoside triphosphate diphosphohydrolase 1 mRNA levels in DPCs were higher than that of the levels identified in the following cell types: MSCs derived from bone marrow, synovium and adipose tissue; and in cells such as FBs, osteoblasts, adipocytes and chondrocytes. The enhanced expression in DPCs was consistently observed irrespective of donor age, tooth type and culture medium. In addition, these genes were expressed at high levels in dental pulp tissue in vivo. In conclusion, this gene set may be useful in the identification and characterization of DPCs in basic studies and pulp cell-based regeneration therapy.