Potential for Chemolithoautotrophy Among Ubiquitous Bacteria Lineages in the Dark Ocean
Potential for Chemolithoautotrophy Among Ubiquitous Bacteria Lineages in the Dark Ocean
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DOI：
10.1126/science.1203690
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发表时间：
2011-09-02
期刊：
影响因子：
56.9
通讯作者：
Stepanauskas, Ramunas
中科院分区：
文献类型：
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作者：
Swan, Brandon K.;Martinez-Garcia, Manuel;Stepanauskas, Ramunas
最近的研究表明，在浩瀚的黑暗海洋中，身份不明的原核生物以全球显著的速度固定无机碳。通过对来自两个亚热带环流的原核生物进行单细胞分选和全基因组扩增，我们从代表大多数世界性血统的738个细胞中获得了基因组DNA。三角洲蛋白细菌群SAR324、伽马蛋白细菌群ARCTIC96BD-19和Agg47的多个细胞以及一些来自中上层下层的海洋螺旋藻含有核酮糖-1，5-二磷酸羧基加氧酶和硫氧化基因。这些结果证实了不同地理区域的群落DNA和RNA图谱。SAR324基因组还提示C(1)代谢和与粒子相关的生活方式。显微放射自显影和荧光原位杂交证实了SAR324细胞对碳酸氢盐的摄取和颗粒结合。我们的研究表明，在黑暗的充氧海洋中普遍存在的几种未培养的变形杆菌谱系中存在潜在的化学自养现象，并为海洋最大栖息地的碳循环提供了新的视角。
Recent studies suggest that unidentified prokaryotes fix inorganic carbon at globally significant rates in the immense dark ocean. Using single-cell sorting and whole-genome amplification of prokaryotes from two subtropical gyres, we obtained genomic DNA from 738 cells representing most cosmopolitan lineages. Multiple cells of Deltaproteobacteria cluster SAR324, Gammaproteobacteria clusters ARCTIC96BD-19 and Agg47, and some Oceanospirillales from the lower mesopelagic contained ribulose-1,5-bisphosphate carboxylase-oxygenase and sulfur oxidation genes. These results corroborated community DNA and RNA profiling from diverse geographic regions. The SAR324 genomes also suggested C(1) metabolism and a particle-associated life-style. Microautoradiography and fluorescence in situ hybridization confirmed bicarbonate uptake and particle association of SAR324 cells. Our study suggests potential chemolithoautotrophy in several uncultured Proteobacteria lineages that are ubiquitous in the dark oxygenated ocean and provides new perspective on carbon cycling in the ocean's largest habitat.