Identification and Biosynthesis of an Aggregation Pheromone of the Storage Mite Chortoglyphus arcuatus
Identification and Biosynthesis of an Aggregation Pheromone of the Storage Mite Chortoglyphus arcuatus
复制标题
贮螨Chortoglyphus arcuatus聚集信息素的鉴定及生物合成
作者：
S. Schulz;Jens Fuhlendorff;J. Steidle;J. Collatz;J. Franz
为了从螨类中鉴定新的信息素，通过使用闭环剥离装置（CLSA）或固相微萃取（SPME），通过GC-MS分析了储藏螨Chortoglyphus acuatus的未受干扰菌落的顶空。螨释放的主要化合物为（4 R，6 R，8 R）-4，6，8-三甲基癸烷-2-酮（4 R，6 R，8 R-8）。通过质谱分析、真实样品合成和手性相气相色谱法解析结构。生物测定表明，这种化合物，我们建议平凡的名字chortolure，是一种聚集信息素，这一物种的两性。几种相关的化合物被螨释放出少量。这些螨的报警信息素，橙花醛和香叶醛，只能在螨的总提取物中找到，其中8只出现在微量。因此，采样方法对于信息素识别至关重要。用氘代丙酸盐进行的饲喂实验表明，chortolure是一种聚酮化合物，通过将四个丙酸盐单元连续加入乙酸盐发酵剂而形成。
In an effort to identify new pheromones from mites, the headspace of undisturbed colonies of the storage mite Chortoglyphus arcuatus was analyzed by GC‐MS by use of a closed‐loop stripping apparatus (CLSA) or solid‐phase microextraction (SPME). The major compound emitted from the mites is (4R,6R,8R)‐4,6,8‐trimethyldecan‐2‐one (4R,6R,8R‐8). The structure was elucidated by analysis of the mass spectrum, synthesis of authentic samples, and gas chromatography on a chiral phase. Bioassays show that this compound, for which we propose the trivial name chortolure, is an aggregation pheromone for both sexes of this species. Several related compounds are released in smaller amounts by the mites. The alarm pheromones of these mites, neral and geranial, can only be found in total extracts of the mites, in which 8 occurs only in minute amounts. The method of sampling is therefore crucial for pheromone identification. Feeding experiments with deuterated propionate showed that chortolure is a polyketide, formed by successive addition of four propionate units to an acetate starter.
DOI：
10.1073/pnas.92.1.44
发表时间：
1995-01-03
影响因子：
11.1
作者：
ROELOFS, WL
通讯作者：
ROELOFS, WL