Transcriptome Landscape of Intracellular Brucella ovis Surviving in RAW264.7 Macrophage Immune System
Transcriptome Landscape of Intracellular Brucella ovis Surviving in RAW264.7 Macrophage Immune System
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RAW264.7 巨噬细胞免疫系统中存活的细胞内绵羊布鲁氏菌的转录组景观
DOI：
10.1007/s10753-020-01239-4
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发表时间：
2020-05-19
期刊：
影响因子：
5.1
通讯作者：
Huang, Qingzhou
中科院分区：
文献类型：
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作者：
Jiao, Hanwei;Li, Bowen;Huang, Qingzhou
绵羊感染布鲁氏菌可导致生殖器官损伤和附睾炎，母羊可导致胎盘炎症和罕见流产，羔羊可导致新生儿死亡。然而，卵巢双歧杆菌感染的机制尚不清楚。在本研究中，我们采用原核转录组测序的方法鉴定了野生型和细胞内型羊双歧杆菌在RAW264.7巨噬细胞中的差异表达基因（DEGs）。进行基因本体（GO）术语富集和京都基因与基因组百科全书（KEGG）途径分析，并使用定量逆转录酶PCR （qRT-PCR）验证前10位上调和下调的deg。结果显示，共有212个基因存在差异表达，其中上调基因68个，下调基因144个，主要富集在与生物过程、细胞成分和分子功能相关的30个氧化石墨烯术语中。KEGG分析显示，DEGs富集于缺氧诱导因子1 （HIF-1）信号通路、丝裂原活化蛋白激酶（MAPK）信号通路、β -丙氨酸代谢通路和群体感应通路。BME_RS01160、BME_RS04270、BME_RS08185、BME_RS12880、BME_RS25875、predicted_RNA865、predicted_RNA953的转录组测序数据得到确认。因此，我们的研究结果不仅揭示了巨噬细胞免疫系统中B. ovis的细胞内寄生，而且有助于了解慢性B. ovis感染的机制。
Brucella ovis infection results in genital damage and epididymitis in rams, placental inflammation and rare abortion in ewes, and neonatal mortality in lambs. However, the mechanism underlying B. ovis infection remains unclear. In the present study, we used prokaryotic transcriptome sequencing to identify the differentially expressed genes (DEGs) between wild-type B. ovis and intracellular B. ovis in RAW264.7 macrophages. Gene ontology (GO) term enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed, and quantitative reverse transcriptase PCR (qRT-PCR) was used to validate the top 10 upregulated and downregulated DEGs. The results showed that 212 genes were differentially expressed, including 68 upregulated and 144 downregulated genes, which were mainly enriched in 30 GO terms linked to biological process, cellular component, and molecular function. KEGG analysis showed that the DEGs were enriched in the hypoxia-inducible factor 1 (HIF-1) signaling pathway, mitogen-activated protein kinase (MAPK) signaling pathway, beta-alanine metabolism, and quorum sensing pathway. BME_RS01160, BME_RS04270, BME_RS08185, BME_RS12880, BME_RS25875, predicted_RNA865, and predicted_RNA953 were confirmed with the transcriptome sequencing data. Hence, our findings not only reveal the intracellular parasitism of B. ovis in the macrophage immune system, but also help to understand the mechanism of chronic B. ovis infection.