Comparative analysis of hemocyte phagocytosis between six species of arthropods as measured by flow cytometry
Comparative analysis of hemocyte phagocytosis between six species of arthropods as measured by flow cytometry
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DOI：
10.1016/j.jip.2011.07.004
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发表时间：
2011-10-01
影响因子：
3.4
通讯作者：
Bartholomay, Lyric
中科院分区：
文献类型：
--
作者：
Oliver, Jonathan D.;Loy, J. Dusty;Bartholomay, Lyric
血细胞对病原体的吞噬作用是一种快速作用的免疫反应，是限制某些节肢动物微生物感染的主要手段。调查的相对能力的血细胞吞噬功能的节肢动物的免疫反应的函数，我们研究了广泛的分类范围的节肢动物物种，包括十足类甲壳动物（凡纳滨对虾），三个硬蜱物种（Amblyomma americanum，Dermacentor variabilis，和Ixodes scapularis），蚊子物种（埃及伊蚊），和幼虫蛾（天蛾）之间的吞噬作用的程度。注射的荧光珠被用作引发吞噬作用的模型，并通过流式细胞术进行测量，详细提供了一种可适用于任何种类的节肢动物的技术。这些数据表明，较小的节肢动物通常比较大的节肢动物具有更高比例的吞噬细胞。(C)2011 Elsevier Inc. All rights reserved.
Phagocytosis of pathogens by hemocytes is a rapid-acting immune response and represents a primary means of limiting microbial infection in some species of arthropods. To survey the relative capacity of hemocyte phagocytosis as a function of the arthropod immune response, we examined the extent of phagocytosis among a wide taxonomic range of arthropod species including a decapod crustacean (Litopenaeus vannamei), three ixodid tick species (Amblyomma americanum, Dermacentor variabilis, and lxodes scapularis), a mosquito species (Aedes aegypti), and a larval moth (Manduca sexta). Injected fluorescent beads were used as a model to elicit phagocytosis and were measured by flow cytometry, a technique provided in detail that may be adapted for use with any species of arthropod. The data indicated that smaller arthropods generally had a higher proportion of phagocytic cells than larger arthropods. (C) 2011 Elsevier Inc. All rights reserved.