Fabrication and characterization of a biphasic scaffold for osteochondral tissue engineering
Fabrication and characterization of a biphasic scaffold for osteochondral tissue engineering
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骨软骨组织工程双相支架的制备和表征
DOI：
10.1016/j.matlet.2011.04.013
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发表时间：
2011-07-15
影响因子：
3
通讯作者：
Yuan, Zhi
中科院分区：
文献类型：
--
作者：
Bi, Long;Li, Dan;Yuan, Zhi
研制了一种具有分层结构的骨软骨组织工程双相支架。软骨相为胶原-壳聚糖复合物。骨相为生物活性玻璃和胶原的复合物。整合在两个相应相中的胶原通过交联剂连接。支架的两层均显示出互连的多孔结构。浸泡在模拟体液中后，骨相表面出现球状颗粒沉淀，经X射线衍射和傅立叶变换红外光谱分析，证明该沉淀为羟基磷灰石。倒置显微镜、荧光显微镜和扫描电镜进一步证实了骨髓基质细胞能够锚在该支架上健康铺展。因此，这种双相支架可能具有作为骨软骨组织工程替代品的巨大潜力。(C)2011 Elsevier B. V.保留所有权利。
A biphasic scaffold with a stratified structure for osteochondral tissue engineering was developed. The chondral phase was a collagen-chitosan composite. The osseous phase was a composite of bioactive glass and collagen. Collagen integrated in the two respective phases was connected by cross-linking agents. Both layers of the scaffold showed interconnected porous structures. After being immersed into stimulated body fluid, precipitation of spherulitic grains could be found on the surface of the osseous phase and this precipitation was proved to be hydroxyapatite by X-ray diffraction and Fourier transform infrared spectroscopy. Inversion, fluorescence and scanning electron microscopy further confirmed that bone marrow stromal cells could anchor on this scaffold with healthy spreading. As the consequence, this biphasic scaffold may have significant potential as an alternative for osteochondral tissue engineering. (C) 2011 Elsevier B.V. All rights reserved.