Microbiome-derived acidity protects against microbial invasion in Drosophila.
Microbiome-derived acidity protects against microbial invasion in Drosophila.
复制标题
微生物组衍生的酸度可以保护果蝇免受微生物入侵。
DOI：
10.1016/j.celrep.2024.114087
复制
发表时间：
2024
期刊：
影响因子：
8.8
通讯作者：
Broderick,NicholeA
中科院分区：
文献类型：
--
作者：
Barron,AlexanderJ;Agrawal,Sneha;Lesperance,DanielleNA;Doucette,Jeremy;Calle,Sthefany;Broderick,NicholeA
微生物入侵是宿主-微生物相互作用的基础，导致发病机制和益生菌定植。在这项研究中，我们探讨了微生物组对果蝇微生物入侵的影响。我们证明了肠道微生物植物乳杆菌和热带醋酸杆菌可以提高存活率，并减少感染过程中的微生物负担。使用微生物相互作用试验，我们报告说，L. plantarum抑制入侵细菌的生长，而A. tropicalis减少这种抑制。我们进一步表明，植物乳杆菌的抑制作用与其通过乳酸脱氢酶产生乳酸来酸化环境的能力有关，而热带曲霉则通过淬灭酸来减弱抑制作用。我们认为，来自微生物组的酸是微生物入侵的看门人，因为只有能够耐受酸性环境的微生物才能在宿主中定居。本文所述的方法和发现将增加在广泛背景下研究微生物-微生物相互作用的工具的广度。
Microbial invasions underlie host-microbe interactions resulting in pathogenesis and probiotic colonization. In this study, we explore the effects of the microbiome on microbial invasion inDrosophila melanogaster. We demonstrate that gut microbesLactiplantibacillus plantarumandAcetobacter tropicalisimprove survival and lead to a reduction in microbial burden during infection. Using a microbial interaction assay, we report thatL. plantaruminhibits the growth of invasive bacteria, whileA. tropicalisreduces this inhibition. We further show that inhibition byL. plantarumis linked to its ability to acidify its environment via lactic acid production by lactate dehydrogenase, whileA. tropicalisdiminishes the inhibition by quenching acids. We propose that acid from the microbiome is a gatekeeper to microbial invasions, as only microbes capable of tolerating acidic environments can colonize the host. The methods and findings described herein will add to the growing breadth of tools to study microbe-microbe interactions in broad contexts.