Sequence and length dependent thermodynamic differences in heterocyclic diamidine interactions at AT base pairs in the DNA minor groove
Sequence and length dependent thermodynamic differences in heterocyclic diamidine interactions at AT base pairs in the DNA minor groove
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DOI：
10.1016/j.bpc.2007.08.007
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发表时间：
2007-12-01
影响因子：
3.8
通讯作者：
Wilson, W. David
中科院分区：
文献类型：
--
作者：
Liu, Yang;Kumar, Arvind;Wilson, W. David
为了更好地了解131375的抗寄生虫生物学作用，我们评价了它与双链交替和非交替序列AT聚合物和低聚物的相互作用。这些DNA在详细的热力学分析中提供了一对重要的序列，分析了DB75与AT位点相互作用的变化。DB75与交替和非交替AT序列结合的结果在基本热力学水平上有很大不同。虽然吉布斯能相近，但DB75与聚(Da)-聚(Dt)和聚(da-dt)-聚(da-dt)的结合热分别为+3.1和-4.5千卡/摩尔，结合熵分别为41.7和15.2caUmol中心点K。与小沟中AT位点结合的热力学在识别过程中起着关键作用。还观察到DB75与Poly(Da)-Poly(DT)结合可以诱导T-A-T三联体的形成，并且该化合物与DT-da-DT三联体结合很强。(C)2007 Elsevier B.V.保留所有权利。
With the goal of developing a better understanding of the antiparasitic biological action of 131375, we have evaluated its interaction with duplex alternating and nonaltemating sequence AT polymers and oligomers. These DNAs provide an important pair of sequences in a detailed thermodynamic analysis of variations in interaction of DB75 with AT sites. The results for DB75 binding to the alternating and nonalternating AT sequences are quite different at the fundamental thermodynamic level. Although the Gibbs energies are similar, the enthalpies for DB75 binding with poly(dA)-poly(dT) and poly(dA-dT)-poly(dA-dT) are +3.1 and -4.5 kcal/mol, respectively, while the binding entropies are 41.7 and 15.2 caUmol center dot K, respectively. The underlying thermodynamics of binding to AT sites in the minor groove plays a key role in the recognition process. It was also observed that DB75 binding with poly(dA)-poly(dT) can induce T-A-T triplet formation and the compound binds strongly to the dT-dA-dT triplex. (c) 2007 Elsevier B.V. All rights reserved.